Enhancement of adhesion and promotion of osteogenic differentiation of human adipose stem cells by poled electroactive poly(vinylidene fluoride)
Jenita Pärssinen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Fimlab Laboratories Ltd., Tampere, 33520 Finland
Search for more papers by this authorHenrik Hammarén
BioMediTech, University of Tampere, Tampere, 33014 Finland
Search for more papers by this authorRolle Rahikainen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Fimlab Laboratories Ltd., Tampere, 33520 Finland
Search for more papers by this authorVitor Sencadas
Center/Department of Physics, University of Minho, Braga, 4710-057 Portugal
Instituto Politécnico do Cávado e do Ave, Campus do IPCA, Barcelos, 4750-810 Portugal
Search for more papers by this authorClarisse Ribeiro
Center/Department of Physics, University of Minho, Braga, 4710-057 Portugal
Search for more papers by this authorSari Vanhatupa
BioMediTech, University of Tampere, Tampere, 33014 Finland
Search for more papers by this authorSusanna Miettinen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Search for more papers by this authorSenentxu Lanceros-Méndez
Center/Department of Physics, University of Minho, Braga, 4710-057 Portugal
Search for more papers by this authorCorresponding Author
Vesa P. Hytönen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Fimlab Laboratories Ltd., Tampere, 33520 Finland
Correspondence to: V. P. Hytönen; e-mail: [email protected]Search for more papers by this authorJenita Pärssinen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Fimlab Laboratories Ltd., Tampere, 33520 Finland
Search for more papers by this authorHenrik Hammarén
BioMediTech, University of Tampere, Tampere, 33014 Finland
Search for more papers by this authorRolle Rahikainen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Fimlab Laboratories Ltd., Tampere, 33520 Finland
Search for more papers by this authorVitor Sencadas
Center/Department of Physics, University of Minho, Braga, 4710-057 Portugal
Instituto Politécnico do Cávado e do Ave, Campus do IPCA, Barcelos, 4750-810 Portugal
Search for more papers by this authorClarisse Ribeiro
Center/Department of Physics, University of Minho, Braga, 4710-057 Portugal
Search for more papers by this authorSari Vanhatupa
BioMediTech, University of Tampere, Tampere, 33014 Finland
Search for more papers by this authorSusanna Miettinen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Search for more papers by this authorSenentxu Lanceros-Méndez
Center/Department of Physics, University of Minho, Braga, 4710-057 Portugal
Search for more papers by this authorCorresponding Author
Vesa P. Hytönen
BioMediTech, University of Tampere, Tampere, 33014 Finland
Fimlab Laboratories Ltd., Tampere, 33520 Finland
Correspondence to: V. P. Hytönen; e-mail: [email protected]Search for more papers by this authorAbstract
Poly(vinylidene fluoride) (PVDF) is a biocompatible material with excellent electroactive properties. Nonelectroactive α-PVDF and electroactive β-PVDF were used to investigate the substrate polarization and polarity influence on the focal adhesion (FA) size and number as well as on human adipose stem cells (hASCs) differentiation. hASCs were cultured on different PVDF surfaces adsorbed with fibronectin and FA size and number, total adhesion area, cell size, cell aspect ratio and FA density were estimated using cells expressing vinculin fused to enhanced green fluorescent protein. Osteogenic differentiation was also determined using a quantitative alkaline phosphatase assay. The surface charge of the poled PVDF films (positive or negative) influenced the hydrophobicity of the samples, leading to variations in the conformation of adsorbed extracellular matrix proteins, which ultimately modulated the stem cell adhesion on the films and induced their osteogenic differentiation. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 919–928, 2015.
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