Improved hMSC functions on titanium coatings by type I collagen immobilization
Haiyong Ao
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Shanghai, China, 200050
Search for more papers by this authorYoutao Xie
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Shanghai, China, 200050
Search for more papers by this authorHonglue Tan
Shanghai Key Laboratory of Orthopedic Implant, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China, 200011
Search for more papers by this authorXiaodong Wu
Department of Orthopedics, Changzheng Hospital, Second Military Medical University, China, 200003
Search for more papers by this authorGuangwang Liu
Department of Orthopedic Surgery, The Central Hospital of Xuzhou, Affiliated Hospital of Medical College of Southeast University, China, 221009
Search for more papers by this authorAn Qin
Centre for Orthopedic Research, School of Surgery, The University of Western Australia, Western Australia, Australia
Search for more papers by this authorCorresponding Author
Xuebin Zheng
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Shanghai, China, 200050
Correspondence to: X. Zheng; e-mail: [email protected] or T. Tang; e-mail: [email protected]Search for more papers by this authorTingting Tang
Shanghai Key Laboratory of Orthopedic Implant, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China, 200011
Search for more papers by this authorHaiyong Ao
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Shanghai, China, 200050
Search for more papers by this authorYoutao Xie
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Shanghai, China, 200050
Search for more papers by this authorHonglue Tan
Shanghai Key Laboratory of Orthopedic Implant, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China, 200011
Search for more papers by this authorXiaodong Wu
Department of Orthopedics, Changzheng Hospital, Second Military Medical University, China, 200003
Search for more papers by this authorGuangwang Liu
Department of Orthopedic Surgery, The Central Hospital of Xuzhou, Affiliated Hospital of Medical College of Southeast University, China, 221009
Search for more papers by this authorAn Qin
Centre for Orthopedic Research, School of Surgery, The University of Western Australia, Western Australia, Australia
Search for more papers by this authorCorresponding Author
Xuebin Zheng
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Shanghai, China, 200050
Correspondence to: X. Zheng; e-mail: [email protected] or T. Tang; e-mail: [email protected]Search for more papers by this authorTingting Tang
Shanghai Key Laboratory of Orthopedic Implant, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China, 200011
Search for more papers by this authorAbstract
In this study, type I collagen was fixed onto plasma-sprayed porous titanium coatings by either adsorptive immobilization or covalent immobilization. Surface characterization by scanning electron microscopy (SEM), diffuse reflectance Fourier transform infrared spectroscopy (DR-FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the biochemical modification of the titanium coatings. The immobilizing effects of type I collagen, including variations in the amount and stability of collagen, were investigated using Sirius red staining. A greater amount of collagen was found on the covalently immobilized titanium coating, and higher stability was achieved relative to the absorptive immobilization surface. Human mesenchymal stem cells (hMSCs) were used to evaluate the cytocompatibility of the modified titanium coatings. Type I collagen immobilized on titanium coating led to enhance cell-material interactions and improved hMSC functions, such as attachment, proliferation, and differentiation. Interestingly, covalently immobilized collagen on titanium coating showed a greater capability to regulate the osteogenic activity of hMSCs than did absorbed collagen, which was explained in terms of the increased amount and higher stability of the covalently linked collagen. The type I collagen covalently immobilized titanium coatings with improved biological function may exhibit better osteointegration in clinical application. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 204–214, 2014.
REFERENCES
- 1Long M, Rack HJ. Titanium alloys in total joint replacement—A materials science perspective. Biomaterials 1998; 19: 1621–1639.
- 2Jaeggi C, Mooser R, Frauchiger V, Wyss P. 3D characterization of open porous vacuum plasma sprayed titanium coatings by means of high resolution micro computer tomography. Mater Lett 2009; 63: 2643–2645.
- 3Treves C, Martinesi M, Stio M, Gutierrez A, Jimenez JA, Lopez MF. In vitro biocompatibility evaluation of surface-modified titanium alloys. J Biomed Mater Res A 2010; 92: 1623–1634.
- 4Xie YT, Liu XY, Zheng XB, Ding CX. Bioconductivity of plasma sprayed dicalcium silicate/titanium composite coatings on Ti-6Al-4V alloy. Surf Coat Tech 2005; 199: 105–111.
- 5Bloebaum RD, Beeks D, Dorr LD, Savory CG, Dupont JA, Hofmann AA. Complications with hydroxyapatite particulate separation in total hip-arthroplasty. Clin Orthop Relat R 1994: 19–26.
- 6Shi J, Ding C, Wu Y. Biomimetic apatite layers on plasma-sprayed titanium coatings after surface modification. Surf Coat Tech 2001; 137: 97–103.
- 7Chen Y, Zheng X, Ji H, Ding C. Effect of Ti–OH formation on bioactivity of vacuum plasma sprayed titanium coating after chemical treatment. Surf Coat Tech 2007; 202: 494–498.
- 8Xue W, Liu X, Zheng X, Ding C. In vivo evaluation of plasma-sprayed titanium coating after alkali modification. Biomaterials 2005; 26: 3029–3037.
- 9Puleo DA, Nanci A. Understanding and controlling the bone–implant interface. Biomaterials 1999; 20( 23–24): 2311–2321.
- 10Mizuno M, Fujisawa R, Kuboki Y. Type I collagen-induced osteoblastic differentiation of bone-marrow cells mediated by collagen-alpha 2 beta 1 integrin interaction. J Cell Physiol 2000; 184: 207–213.
- 11Müller R, Abke J, Schnell E, Scharnweber D, Kujat R, Englert C, Taheri D, Nerlich M, Angele P. Influence of surface pretreatment of titanium- and cobalt-based biomaterials on covalent immobilization of fibrillar collagen. Biomaterials 2006; 27: 4059–4068.
- 12Wang QQ, Li W, Yang BC. Regulation on the biocompatibility of bioactive titanium metals by type I collagen. J Biomed Mater Res A 2011; 99: 125–134.
- 13Morra M, Cassinelli C, Cascardo G, Bollati D, Baena RRY. Gene expression of markers of osteogenic differentiation of human mesenchymal cells on collagen I-modified microrough Titanium surfaces. J Biomed Mater Res A 2011; 96: 449–455.
- 14Becker D, Geißler U, Hempel U, Bierbaum S, Scharnweber D, Worch H, Wenzel KW. Proliferation and differentiation of rat calvarial osteoblasts on type I collagen-coated titanium alloy. J Biomed Mater Res 2002; 59: 516–527.
- 15Jonge LT, Leeuwenburgh SCG, Wolke JGC, Jansen JA. Organic–inorganic surface modifications for titanium implant surfaces. Pharmaceutical Res 2008; 25: 2357–2369.
- 16Porté-Durrieu MC, Guillemot F, Pallu S, Labrugère C, Brouillaud B, Bareille R, Amédée J, Barthe N, Dard M, Baquey C. Cyclo-(DfKRG) peptide grafting onto Ti–6Al–4V: Physical characterization and interest towards human osteoprogenitor cells adhesion. Biomaterials 2004; 25: 4837–4846.
- 17Müller R, Abke J, Schnell E, Macionczyk F, Gbureck U, Mehrl R, Ruszczak Z, Kujat R, Englert C, Nerlich M, et al. Surface engineering of stainless steel materials by covalent collagen immobilization to improve implant biocompatibility. Biomaterials 2005; 26: 6962–6972.
- 18Li B, Liu X, Cao C, Ding C. Biocompatibility and antibacterial activity of plasma sprayed titania coating grafting collagen and gentamicin. J Biomed Mater Res A 2007; 83: 923–930.
- 19Angele P, Abke J, Kujat R, Faltermeier H, Schumann D, Nerlich M, Kinner B, Englert C, Ruszczak Z, Mehrl R, et al. Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices. Biomaterials 2004; 25: 2831–2841.
- 20Peng Z-X, Wang L, Du L, Guo S-R, Wang X-Q, Tang T-T. Adjustment of the antibacterial activity and biocompatibility of hydroxypropyltrimethyl ammonium chloride chitosan by varying the degree of substitution of quaternary ammonium. Carbohyd Polym 2010; 81: 275–283.
- 21Tan H, Guo S, Yang S, Xu X, Tang T. Physical characterization and osteogenic activity of the quaternized chitosan-loaded PMMA bone cement. Acta Biomater 2012; 8: 2166–2174.
- 22Yang F, Xie Y, Li H, Tang T, Zhang X, Gan Y, Zheng X, Dai K. Human bone marrow-derived stromal cells cultured with a plasma sprayed CaO-ZrO2-SiO2 coating. J Biomed Mater Res B: Appl Biomater 2010; 95: 192–201.
- 23Cai YL, Liang CY, Zhu SL, Cui ZD, Yang XJ. Formation of bonelike apatite-collagen composite coating on the surface of NiTi shape memory alloy. Scripta Mater 2006; 54: 89–92.
- 24Ye S-H, Johnson CA, Woolley JR, Murata H, Gamble LJ, Ishihara K, Wagner WR. Simple surface modification of a titanium alloy with silanated zwitterionic phosphorylcholine or sulfobetaine modifiers to reduce thrombogenicity. Colloid Surf B: Biointerf 2010; 79: 357–364.
- 25Chen JL, Li QL, Chen JY, Chen C, Huang N. Improving blood-compatibility of titanium by coating collagen–heparin multilayers. Appl Surf Sci 2009; 255: 6894–6900.
- 26Kim H-W, Li L-H, Lee E-J, Lee S-H, Kim H-E. Fibrillar assembly and stability of collagen coating on titanium for improved osteoblast responses. J Biomed Mater Res A 2005; 75: 629–638.
- 27Hwal Suh Y-SH, Jong-Eun Lee, Chang Dong Han, Jong-Chul Park. Behavior of osteoblasts on a type I atelocollagen grafted ozone oxidized poly-lactic acid membrane. Biomaterials 2001; 22: 219–230.
- 28Hu X, Neoh K-G, Shi Z, Kang E-T, Poh C, Wang W. An in vitro assessment of titanium functionalized with polysaccharides conjugated with vascular endothelial growth factor for enhanced osseointegration and inhibition of bacterial adhesion. Biomaterials 2010; 31: 8854–8863.
- 29Salasznyk RM, Williams WA, Boskey A, Batorsky A, Plopper GE. Adhesion to vitronectin and collagen I promotes osteogenic differentiation of human mesenchymal stem cells. J Biomed Biotechnol 2004; 1: 24–34.
- 30Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli LV. Differentiation of human bone-marrow osteogenic stromal cells in vitro—Induction of the osteoblast phenotype by dexamethasone. Endocrinology 1994; 134: 277–286.
- 31Rickard DJ, Sullivan TA, Shenker BJ, Leboy PS, Kazhdan I. Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2. Dev Biol 1994; 161: 218–228.
- 32Kundu AK, Putnam AJ. Vitronectin and collagen I differentially regulate osteogenesis in mesenchymal stem cells. Biochem Bioph Res Co 2006; 347: 347–357.
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