Evaluation of cell affinity on poly(L-lactide) and poly(ε-caprolactone) blends and on PLLA-b-PCL diblock copolymer surfaces
Corresponding Author
Diana Ajami-Henriquez
Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Diana Ajami-Henriquez, Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Alejandro J. Müller, Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorMónica Rodríguez
Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorMarcos Sabino
Departamento de Química, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorR. Verónica Castillo
Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorCorresponding Author
Alejandro J. Müller
Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Diana Ajami-Henriquez, Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Alejandro J. Müller, Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorAdriana Boschetti-de-Fierro
Institute of Polymer Research, GKSS Research Centre Geesthacht GmbH, 21502 Geesthacht, Germany
Search for more papers by this authorClarissa Abetz
Institute of Polymer Research, GKSS Research Centre Geesthacht GmbH, 21502 Geesthacht, Germany
Search for more papers by this authorVolker Abetz
Institute of Polymer Research, GKSS Research Centre Geesthacht GmbH, 21502 Geesthacht, Germany
Search for more papers by this authorPhilippe Dubois
Laboratory of Polymeric and Composite Materials (LPCM), University of Mons-Hainaut, Place du Parc 20, 7000 Mons, Belgium
Search for more papers by this authorCorresponding Author
Diana Ajami-Henriquez
Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Diana Ajami-Henriquez, Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Alejandro J. Müller, Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorMónica Rodríguez
Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorMarcos Sabino
Departamento de Química, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorR. Verónica Castillo
Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorCorresponding Author
Alejandro J. Müller
Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Diana Ajami-Henriquez, Departamento de Biología Celular, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Alejandro J. Müller, Grupo de Polímeros USB, Departamento de Ciencia de los Materiales, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
Search for more papers by this authorAdriana Boschetti-de-Fierro
Institute of Polymer Research, GKSS Research Centre Geesthacht GmbH, 21502 Geesthacht, Germany
Search for more papers by this authorClarissa Abetz
Institute of Polymer Research, GKSS Research Centre Geesthacht GmbH, 21502 Geesthacht, Germany
Search for more papers by this authorVolker Abetz
Institute of Polymer Research, GKSS Research Centre Geesthacht GmbH, 21502 Geesthacht, Germany
Search for more papers by this authorPhilippe Dubois
Laboratory of Polymeric and Composite Materials (LPCM), University of Mons-Hainaut, Place du Parc 20, 7000 Mons, Belgium
Search for more papers by this authorAbstract
An evaluation of cell proliferation and adhesion on biocompatible film supports was performed. A series of films were compression molded from commercially available poly (L-lactide), PLLA, and poly(ε-caprolactone), PCL, and from their melt mixed blends (PLLA/PCL blends). These were compared with compression molded films of PLLA-b-PCL model diblock copolymers. The samples were analyzed by differential scanning calorimetry (DSC), contact angle measurements, and scanning force microscopy (SFM). Cell adhesion and proliferation were performed with monkey derived fibroblasts (VERO) and with osteoblastic cells obtained either enzymatically or from explants cultures of Sprague–Dawley rat calvaria. Migration studies were performed with bone explants of the same origin. The results obtained indicate that although all materials tested were suitable for the support of cellular growth, a PLLA-b-PCL diblock copolymer sample with 93% PLLA was significantly more efficient. This sample exhibited a unique surface morphology with long range ordered domains (of the order of 2–3 μm) of edge-on PLLA lamellae that can promote “cell contact guidance.” The influence of other factors such as chemical composition, degree of crystallinity, and surface roughness did not play a major role in determining cell preference toward a specific surface for the materials employed in this work. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
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