Corrosive effects of fluoride on titanium: Investigation by X-ray photoelectron spectroscopy, atomic force microscopy, and human epithelial cell culturing
Anette Stájer
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorKrisztina Ungvári
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorIstván K. Pelsőczi
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorHilda Polyánka
Dermatological Research Group of the Hungarian Academy of Sciences, Korányi fasor 6-8, 6720 Szeged, Hungary
Search for more papers by this authorAlbert Oszkó
Department of Solid-State and Radiochemistry, University of Szeged, Aradi vértanúk tere 1, 6720 Szeged, Hungary
Search for more papers by this authorErzsébet Mihalik
Department of Botany and Botanic Garden, University of Szeged, Egyetem u.2, 6721 Szeged, Hungary
Search for more papers by this authorZoltán Rakonczay
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorMárta Radnai
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorLajos Kemény
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6-8, 6720 Szeged, Hungary
Search for more papers by this authorAndrás Fazekas
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorCorresponding Author
Kinga Turzó
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, HungarySearch for more papers by this authorAnette Stájer
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorKrisztina Ungvári
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorIstván K. Pelsőczi
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorHilda Polyánka
Dermatological Research Group of the Hungarian Academy of Sciences, Korányi fasor 6-8, 6720 Szeged, Hungary
Search for more papers by this authorAlbert Oszkó
Department of Solid-State and Radiochemistry, University of Szeged, Aradi vértanúk tere 1, 6720 Szeged, Hungary
Search for more papers by this authorErzsébet Mihalik
Department of Botany and Botanic Garden, University of Szeged, Egyetem u.2, 6721 Szeged, Hungary
Search for more papers by this authorZoltán Rakonczay
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorMárta Radnai
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorLajos Kemény
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6-8, 6720 Szeged, Hungary
Search for more papers by this authorAndrás Fazekas
Department of Prosthetic Dentistry, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Search for more papers by this authorCorresponding Author
Kinga Turzó
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, Hungary
Department of Oral Biology, Faculty of Dentistry, University of Szeged, Tisza Lajos krt.64, 6720 Szeged, HungarySearch for more papers by this authorAbstract
High fluoride (F−) concentrations and acidic pH impair the corrosion resistance of titanium (Ti). Effects of F−-containing caries-preventive prophylactic rinses, and gels on Ti were investigated by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Human epithelial cell attachment and proliferation were investigated by dimethylthiazol-diphenyl tetrazolium bromide (MTT) and protein content assays. Aqueous 1% NaF solution (3800 ppm F−, pH 4.5) or high (12,500 ppm) F− content gel (pH 4.8) strongly corroded the surface and modified its composition. XPS revealed formation of a strongly bound F−-containing complex (Na2TiF6). AFM indicated an increase in roughness (Ra) of the surfaces: 10-fold for the NaF solution and smaller for the gel or a mouthwash (250 ppm F−, pH 4.4). MTT revealed that cell attachment was significantly increased by the gel, but was not disturbed by either the mouthwash or the NaF. Cell proliferation determined by MTT decreased significantly only for the NaF-treated samples; protein content assay experiments showed no such effect. This study indicates that epithelial cell culturing results can depend on the method used, and the adverse effects of a high F− concentration and low pH should be considered when prophylactic gels are applied by patients with Ti implants or other dental devices. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
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