Multistep process of neoplastic transformation of normal human fibroblasts by 60CO gamma rays and harvey sarcoma viruses
Corresponding Author
Masayoshi Namba
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, JapanSearch for more papers by this authorKoji Nishitani
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Search for more papers by this authorFujiko Fukushima
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Search for more papers by this authorTetsuo Kimoto
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Search for more papers by this authorKiyoshi Nose
Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorCorresponding Author
Masayoshi Namba
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, JapanSearch for more papers by this authorKoji Nishitani
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Search for more papers by this authorFujiko Fukushima
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Search for more papers by this authorTetsuo Kimoto
Department of Pathology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701–01, Japan
Search for more papers by this authorKiyoshi Nose
Department of Cancer Cell Research, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Search for more papers by this authorAbstract
As reported previously (Namba et al., 1985), normal human fibroblasts were transformed by 60Co gamma-ray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST-6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha-MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha-MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha-MSV-transformed KMST-6 cells showed an enhanced expression of the ras oncogene, but normal and 60Co gamma-ray-transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha-MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one showing enhanced colony formation in soft agar and tumorigenicity in nude mice.
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