Experimental Cancer
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Altered growth properties and cell surface changes in ras transformed mouse bladder epithelium
I. C. Summerhayes,
P. Malone,
K. Visvanathan,
I. C. Summerhayes
Institute of Cancer Research: Royal Cancer Hospital, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Search for more papers by this authorP. Malone
Institute of Cancer Research: Royal Cancer Hospital, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Search for more papers by this authorK. Visvanathan
Institute of Cancer Research: Royal Cancer Hospital, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Search for more papers by this authorI. C. Summerhayes,
P. Malone,
K. Visvanathan,
I. C. Summerhayes
Institute of Cancer Research: Royal Cancer Hospital, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Search for more papers by this authorP. Malone
Institute of Cancer Research: Royal Cancer Hospital, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Search for more papers by this authorK. Visvanathan
Institute of Cancer Research: Royal Cancer Hospital, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Search for more papers by this authorAbstract
Transfection of the c-Ha-ras-1 oncogene, cloned from EJ/T24 cells, into different mouse bladder epithelial cell lines resulted in the acquisition of tumorigenic potential and, in all but one cell line (MB331), anchorage-independent growth. Sera from syngeneic mice bearing tumours immunoprecipitated an 18 kDa protein from ras-transfected urothelial cells which was not detectable in their parental counterparts. Screening of a limited panel of mouse cell lines showed this protein to be urothelium-specific and associated with the expression of an activated ras gene. Polyoma middle T and v-myc-transfected bladder epithelial cells did not express this 18kDa protein. Localization of this protein to the cell surface was demonstrated by immunofluorescence and absorption studies.
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