Volume 25, Issue 6 pp. 705-710
Human Cancer
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Establishment and characterization of a new leukaemic T-cell line (Peer) with an unusual phenotype

Zohar Ravid

Zohar Ravid

Chanock Centre for Virology, Hebrew University-Hadassah Medical School, Jerusalem

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Natan Goldblum

Natan Goldblum

Chanock Centre for Virology, Hebrew University-Hadassah Medical School, Jerusalem

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Rina Zaizov

Rina Zaizov

Department of Pediatric Oncology, Beilinson Hospital, Petach Tikva, Israel

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Michael Schlesinger

Michael Schlesinger

Hubert H. Humphrey Centre for Experimental Medicine and Cancer, Hebrew University-Hadassah Medical School, Jerusalem

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Tamar Kertes

Tamar Kertes

Hubert H. Humphrey Centre for Experimental Medicine and Cancer, Hebrew University-Hadassah Medical School, Jerusalem

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Jun Minowada

Jun Minowada

Department of Immunology, Roswell Park Memorial Institute, Buffalo, N.Y., USA

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Winston Verbi

Winston Verbi

Membrane Immunology Laboratory, Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London, England

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Melvyn Greaves

Melvyn Greaves

Membrane Immunology Laboratory, Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London, England

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First published: 15 June 1980
Citations: 52

Abstract

We report the isolation and establishment in continuous culture of a human lymphoid cell line (Peer) from a case of T-leukemia. The Peer cell line lacks some typical cell-surface properties of T cells, namely sheep erythrocyte rosette formation and reactivity with two anti-T-cell sera, but has focal acid phosphatase and does express two other T-cell antigens, one defined by a monoclonal antibody, the other related to a T-cell subset (TH2). The cells are negative for B-cell markers (Smlg or cytoplasmic μ Fcγ and C3 receptors, mouse erythrocyte rosettes) and EBV (EBNA). In addition, the Peer cell does not possess the typical phenotypic markers of “non-B, non-T” leukemia: cALL and la-like antigens, and the cytoplasmic hexosaminidase isoenzyme I, but is positive for terminal deoxynucleotidyl transferase by enzymatic and immunofluorescent criteria. The cell line requires exogenous L-asparagine for adequate growth in culture, a property known to be characteristic of certain T cells but not of B cells. The Peer cell line appears to have a maturation arrest at a developmental stage intermediate between the cortical thymocyte and a mature T-cell subset and to have lost some T-cell differentiation features.

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