Patchy distribution of mucosal lesions in ileal Crohn's disease is not linked to differences in the dominant mucosa-associated bacteria: A study using fluorescence in situ hybridization and temporal temperature gradient gel electrophoresis†
Nadia Vasquez MS
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Search for more papers by this authorCorresponding Author
Irène Mangin PhD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, FranceSearch for more papers by this authorPatricia Lepage PhD
Unité d'écologie et physiologie du système digestif, INRA Jouy-en-Josas, France
Search for more papers by this authorPhilippe Seksik MD
Département d'Hépato-Gastroentérologie, Hôpital Saint-Antoine, AP-HP, Paris, France
Search for more papers by this authorJean-Paul Duong MD
Service d'anatomopathologie, Hôpital Européen Georges Pompidou, Paris, France
Search for more papers by this authorStéphanie Blum PhD
Nestle Research Center, Vers-Chez-les-Blanc, Switzerland
Search for more papers by this authorEduardo Schiffrin PhD
Nestle Research Center, Vers-Chez-les-Blanc, Switzerland
Search for more papers by this authorAntonia Suau MD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Search for more papers by this authorMatthieu Allez MD
Service d'Hépato-Gastroentérologie, Hôpital Saint Louis, Paris, France
Search for more papers by this authorGwenola Vernier MD
Service des maladies de l'appareil digestif, Hôpital Huriez, CHRU de Lille, France
Search for more papers by this authorXavier Tréton MD
Service de Gastro-Entérologie et Assistance Nutritive, Hôpital Beaujon, Clichy, France
Search for more papers by this authorJoël Doré PhD
Unité d'écologie et physiologie du système digestif, INRA Jouy-en-Josas, France
Search for more papers by this authorPhilippe Marteau MD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Université Paris 5-Descartes, Faculté de médecine, AP-HP, Département Médico-chirurgical de Pathologie Digestive, Hôpital Lariboisière, Paris, France
Search for more papers by this authorPhilippe Pochart PhD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Search for more papers by this authorNadia Vasquez MS
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Search for more papers by this authorCorresponding Author
Irène Mangin PhD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, FranceSearch for more papers by this authorPatricia Lepage PhD
Unité d'écologie et physiologie du système digestif, INRA Jouy-en-Josas, France
Search for more papers by this authorPhilippe Seksik MD
Département d'Hépato-Gastroentérologie, Hôpital Saint-Antoine, AP-HP, Paris, France
Search for more papers by this authorJean-Paul Duong MD
Service d'anatomopathologie, Hôpital Européen Georges Pompidou, Paris, France
Search for more papers by this authorStéphanie Blum PhD
Nestle Research Center, Vers-Chez-les-Blanc, Switzerland
Search for more papers by this authorEduardo Schiffrin PhD
Nestle Research Center, Vers-Chez-les-Blanc, Switzerland
Search for more papers by this authorAntonia Suau MD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Search for more papers by this authorMatthieu Allez MD
Service d'Hépato-Gastroentérologie, Hôpital Saint Louis, Paris, France
Search for more papers by this authorGwenola Vernier MD
Service des maladies de l'appareil digestif, Hôpital Huriez, CHRU de Lille, France
Search for more papers by this authorXavier Tréton MD
Service de Gastro-Entérologie et Assistance Nutritive, Hôpital Beaujon, Clichy, France
Search for more papers by this authorJoël Doré PhD
Unité d'écologie et physiologie du système digestif, INRA Jouy-en-Josas, France
Search for more papers by this authorPhilippe Marteau MD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Université Paris 5-Descartes, Faculté de médecine, AP-HP, Département Médico-chirurgical de Pathologie Digestive, Hôpital Lariboisière, Paris, France
Search for more papers by this authorPhilippe Pochart PhD
Laboratoire de Biologie EA 3199, CNAM, 2 rue Conté 75003 Paris, France
Search for more papers by this authorThis study was part of a larger clinical study initiated, designed, and conducted by the GETAID Study Group. It was funded by grant support from the Nestlé Research Centre, Vevey, Switzerland.
Abstract
Background: The mucosa-associated bacteria (MAB) are suspected of being involved in the pathogenesis of Crohn's disease. We analyzed and compared the MAB in noninflamed and inflamed ileal mucosa of Crohn's disease patients (n = 22).
Methods: Tissue samples from the inflamed ileal mucosa and from the adjacent noninflamed ileal mucosa were taken from surgical resection specimens. The MAB were investigated using fluorescence in situ hybridization with 7 group-specific probes and temporal temperature gradient gel electrophoresis (TTGE).
Results: Samples from both noninflamed and inflamed mucosa were obtained from 15 patients. The distribution of the bacterial populations was not different between noninflamed and inflamed mucosa. The Bacteroidetes phylum was dominant and accounted for 29% of MAB (0%–74%) in noninflamed tissues and 32% (0%–70%) in inflamed areas. The γ Proteobacteria represented 12% (0%–70%) of MAB both in noninflamed and inflamed areas. The Clostridium coccoides group (Firmicutes phylum) represented 15% of MAB in noninflamed tissues versus 7% in inflamed areas. For most of the patients the similarity index between TTGE paired profiles was very high.
Conclusion: The dominant MAB do not differ between noninflamed and inflamed ileal mucosa in Crohn's disease. This argues against a localized dysbiosis to explain the patchy distribution of mucosal lesions.
(Inflamm Bowel Dis 2007)
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