Volume 18, Issue 5 pp. 1039-1044

Original Article

Free Access

HLA B44–restricted cytotoxic T lymphocytes recognizing an epitope on hepatitis C virus nucleocapsid protein

Hiroto Kita,

Hiroto Kita

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Takashi Moriyama,

Takashi Moriyama

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Takashi Kaneko,

Takashi Kaneko

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Ichiro Harase,

Ichiro Harase

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Masayuki Nomura,

Masayuki Nomura

Institute for Life Science Research, Asahi Chemical Industry Co., Ltd., Shizuoka 416, Japan

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Hideaki Miura,

Hideaki Miura

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Ikuo Nakamura,

Ikuo Nakamura

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Yoshio Yazaki,

Yoshio Yazaki

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Michio Imawari M.D.,

Corresponding Author

Michio Imawari M.D.

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

Department of Gastroenterology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan===Search for more papers by this author

Hiroto Kita,

Hiroto Kita

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

Search for more papers by this author

Takashi Moriyama,

Takashi Moriyama

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

Search for more papers by this author

Takashi Kaneko,

Takashi Kaneko

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

Search for more papers by this author

Ichiro Harase,

Ichiro Harase

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

Search for more papers by this author

Masayuki Nomura,

Masayuki Nomura

Institute for Life Science Research, Asahi Chemical Industry Co., Ltd., Shizuoka 416, Japan

Search for more papers by this author

Hideaki Miura,

Hideaki Miura

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Ikuo Nakamura,

Ikuo Nakamura

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Yoshio Yazaki,

Yoshio Yazaki

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

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Michio Imawari M.D.,

Corresponding Author

Michio Imawari M.D.

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113

Department of Gastroenterology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan===Search for more papers by this author

First published: November 1993

https://doi.org/10.1002/hep.1840180504

Citations: 84

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Abstract

Cytotoxic T lymphocytes have been reported to be involved in the immune clearance of virus-infected cells and in the pathogenesis of viral infection. We studied the cytotoxic T lymphocyte response to the putative nucleocapsid protein of hepatitis C virus in patients with chronic hepatitis C. Cytotoxic T lymphocytes specific for hepatitis C virus nucleocapsid protein were generated from peripheral blood lymphocytes by means of repeated stimulation with a synthetic hepatitis C virus nucleocapsid protein peptide. The cytotoxic T lymphocytes were CD8 positive and recognized an epitope in hepatitis C virus nucleocapsid protein residues 81 to 100 in association with a human leukocyte antigen class I molecule, B44. The peptideinduced cytotoxic T lymphocytes recognized target cells synthesizing hepatitis C virus nucleocapsid protein endogenously, though less efficiently than peptide-pulsed target cells. The human leukocyte antigen B44–restricted cytotoxic T lymphocyte response was observed in three of five patients with chronic hepatitis C and a human leukocyte antigen B44 molecule but in neither of two hepatitis C virus–negative healthy individuals with human leukocyte antigen B44 molecules. The results demonstrate the presence of hepatitis C virus–specific cytotoxic T lymphocytes in the peripheral blood of patients with chronic hepatitis C and provide a strategy to study the role of cytotoxic T lymphocytes in the viral clearance and the pathogenesis of hepatitis C virus infection. (HEPATOLOGY 1993;18:1039-1044).

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Volume18, Issue5

November 1993

Pages 1039-1044

HLA B44–restricted cytotoxic T lymphocytes recognizing an epitope on hepatitis C virus nucleocapsid protein

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HLA B44–restricted cytotoxic T lymphocytes recognizing an epitope on hepatitis C virus nucleocapsid protein

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