Immunolpathology of acute galactosamine hepatitis in rats
Corresponding Author
A. Meike Jonker M.D
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Department of Pathology, University of Groningen, Oostersingel 63, 9713 EZ Groningen, The Netherlands===Search for more papers by this authorFreke W. J. Dijkhuis
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorFrans G. M. Kroese
Histology, University of Groaningen, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorMachiel J. Hardonk
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorJoris Grond
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorCorresponding Author
A. Meike Jonker M.D
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Department of Pathology, University of Groningen, Oostersingel 63, 9713 EZ Groningen, The Netherlands===Search for more papers by this authorFreke W. J. Dijkhuis
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorFrans G. M. Kroese
Histology, University of Groaningen, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorMachiel J. Hardonk
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorJoris Grond
Department of Pathology, Oostersingel 63, 9713 EZ Groningen, The Netherlands
Search for more papers by this authorAbstract
Galactosamine hydrochloride induces liver disease in tats that morphologically resembles drug-induced hepatitis in man. In this study we analyzed the character of the inflammatory reaction following the toxic damage resulting from the administration of galactosamine hydrochloride using a broad panel of mono clonal antibodies to lymphocyte subsets and macrophages. Fat-storing cells were identified with a polyclonal anti-desmin antibody. Cellular proliferation was assessed by labeling S-phase cells with the thymidine analog bronmodeoxyuridine. Injection of galactosamine hydrochloride was associated with conspicuous hepatocyte necrosis and parenchymal granulocyte influx in the first 24 hr. thereafter, mononuclear inflammatory cells predominated, mainly T lymphocytes and macorphages, with maximal numbers at 48 hr. The majority of T lymphocytes were CD8-positive cells and were located in the portal tracts and parenchyma. CD4 positive T cells were scarce and confined to the portal tracts. Proliferation of fat-storing cells paralleled hepatocyte regeneration with maximal values after 48 to 72 hr.
The temporal relationship between infiltrating mononuclear cells, mainly T lymphocytes of CD8 phenotype and macrophages, fat-storing cell proliferation and hepatic gegeneration suggests pathophysiological interactions between these cell types in liver injury in the rat after galactosamine hydrochloride administration.(HEPATOLOGY 1990; 11:622:627.)
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