Correlation between podoplanin expression and extracapsular spread in squamous cell carcinoma of the oral cavity using subjective immunoreactivity scores and semiquantitative image analysis
Maxime Mermod MD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorMassimo Bongiovanni MD
Institute of Pathology, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorTatiana V. Petrova PhD
Department of Fundamental Oncology, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorElena A. Dubikovskaya PhD
Laboratory of Bioorganic Chemistry and Molecular Imaging (LBCMI), Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Search for more papers by this authorChristian Simon MD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorGenrich Tolstonog MD, PhD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Genrich Tolstonog and Yan Monnier contributed equally to this work.
Search for more papers by this authorCorresponding Author
Yan Monnier MD, PhD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Corresponding author: Y. Monnier, Department of Otolaryngology – Head and Neck Surgery, CHUV - University Hospital, Rue du Bugnon 46, CH-1011 Lausanne, Switzerland. E-mail: [email protected]Search for more papers by this authorMaxime Mermod MD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorMassimo Bongiovanni MD
Institute of Pathology, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorTatiana V. Petrova PhD
Department of Fundamental Oncology, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorElena A. Dubikovskaya PhD
Laboratory of Bioorganic Chemistry and Molecular Imaging (LBCMI), Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Search for more papers by this authorChristian Simon MD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Search for more papers by this authorGenrich Tolstonog MD, PhD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Genrich Tolstonog and Yan Monnier contributed equally to this work.
Search for more papers by this authorCorresponding Author
Yan Monnier MD, PhD
Department of Otolaryngology – Head and Neck Surgery, Head and Neck Tumor Laboratory, CHUV and University of Lausanne, Lausanne, Switzerland
Corresponding author: Y. Monnier, Department of Otolaryngology – Head and Neck Surgery, CHUV - University Hospital, Rue du Bugnon 46, CH-1011 Lausanne, Switzerland. E-mail: [email protected]Search for more papers by this authorAbstract
Background
The correlation between podoplanin expression and extracapsular spread in head and neck squamous cell carcinoma (HNSCC) has never been reported. The purpose of this study was to assess the predictive value of podoplanin expression for this parameter.
Methods
Subjective immunoreactivity scores and semiquantitative image analysis of podoplanin expression were performed in 67 patients with primary oral squamous cell carcinoma and in their corresponding lymph nodes. Neck classification showed 34 cases (51%) of pN0 and 33 cases (49%) of pN+. Correlation between the levels of podoplanin expression and the histopathological data was established.
Results
In lymph nodes, a high level of podoplanin expression correlated with the presence of extracapsular spread by multivariate analysis (p = .03). A strong correlation between subjective and semiquantitative image analysis was observed (r = 0.77; p < .001).
Conclusion
A high level of podoplanin expression in lymph node metastases of oral squamous cell carcinoma is independently associated with extracapsular spread. © 2016 Wiley Periodicals, Head Neck 39: 98–108, 2017
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