Volume 37, Issue 3 pp. 317-326

Original Article

Cancer stem cells: Mediators of tumorigenesis and metastasis in head and neck squamous cell carcinoma

Steven B. Chinn MD,

Steven B. Chinn MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Owen A. Darr MD,

Owen A. Darr MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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John H. Owen MS,

John H. Owen MS

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Emily Bellile MS,

Emily Bellile MS

Comprehensive Cancer Center Biostatistics Unit, University of Michigan, Ann Arbor, Michigan

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Jonathan B. McHugh MD,

Jonathan B. McHugh MD

Department of Pathology, University of Michigan, Ann Arbor, Michigan

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Matthew E. Spector MD,

Matthew E. Spector MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Silvana M. Papagerakis MD, PhD,

Silvana M. Papagerakis MD, PhD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Douglas B. Chepeha MD,

Douglas B. Chepeha MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Carol R. Bradford MD,

Carol R. Bradford MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Thomas E. Carey PhD,

Thomas E. Carey PhD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Mark E. P. Prince MD,

Corresponding Author

Mark E. P. Prince MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

Corresponding author: M. E. Prince, Department of Otolaryngology – Head and Neck Surgery, University of Michigan, 1500 E. Medical Center Drive, Ann Arbor, MI 48103. E-mail: [email protected]Search for more papers by this author

Steven B. Chinn MD,

Steven B. Chinn MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Owen A. Darr MD,

Owen A. Darr MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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John H. Owen MS,

John H. Owen MS

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Emily Bellile MS,

Emily Bellile MS

Comprehensive Cancer Center Biostatistics Unit, University of Michigan, Ann Arbor, Michigan

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Jonathan B. McHugh MD,

Jonathan B. McHugh MD

Department of Pathology, University of Michigan, Ann Arbor, Michigan

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Matthew E. Spector MD,

Matthew E. Spector MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Silvana M. Papagerakis MD, PhD,

Silvana M. Papagerakis MD, PhD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Douglas B. Chepeha MD,

Douglas B. Chepeha MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Carol R. Bradford MD,

Carol R. Bradford MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Thomas E. Carey PhD,

Thomas E. Carey PhD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

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Mark E. P. Prince MD,

Corresponding Author

Mark E. P. Prince MD

Department of Otolaryngology – Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan

First published: 10 January 2014

https://doi.org/10.1002/hed.23600

Citations: 57

This work was presented at the 8th International Conference on Head and Neck Cancer, Toronto, Ontario, Canada, July 2012.

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ABSTRACT

Background

Cancer stem cells (CSCs) represent a subpopulation of cells responsible for tumor growth. Their role in head and neck squamous cell carcinoma (HNSCC) tumorigenesis and metastasis remains uncertain.

Methods

Wound healing and an orthotopic animal model were used to study cells expressing the CSC phenotype (CD44^high and aldehyde dehydrogenase [ALDH]⁺) and assess mobility, tumorigenesis, and metastasis. A prospective collection of 40 patient-derived primary HNSCC specimens were analyzed for CSC-proportion compared to clinical variables.

Results

CSCs exhibited significantly faster wound closure and greater tumorigenesis and regional metastasis in vivo than non-CSCs. In primary patient tumors, size and advanced stage were correlated with elevated proportion of CSCs, however, not with survival.

Conclusion

HNSCC stem cells mediate tumorigenesis and regional metastasis in vivo. In primary patient tumors, CSC-proportion was associated with tumor size and stage, but not with metastatic spread or survival. CSC burden alone may only represent a minor variable in understanding CSCs and metastasis. © 2014 Wiley Periodicals, Inc. Head Neck 37: 317–326, 2015

Supporting Information

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Volume37, Issue3

March 2015

Pages 317-326

Cancer stem cells: Mediators of tumorigenesis and metastasis in head and neck squamous cell carcinoma

ABSTRACT

Background

Methods

Results

Conclusion

Supporting Information

REFERENCES

Citing Literature

References

Information

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Cancer stem cells: Mediators of tumorigenesis and metastasis in head and neck squamous cell carcinoma

ABSTRACT

Background

Methods

Results

Conclusion

Supporting Information

REFERENCES

Citing Literature

References

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