Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer
Peter Szaniszlo MD, PhD
Department of Otolaryngology, UTMB Health Cancer Center, Galveston, Texas
UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorSusan M. Fennewald PhD
Department of Otolaryngology, UTMB Health Cancer Center, Galveston, Texas
UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorSuimin Qiu MD, PhD
UTMB Health Cancer Center, Galveston, Texas
Department of Pathology, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorCarla Kantara PhD
UTMB Health Cancer Center, Galveston, Texas
Department of Neuroscience and Cell Biology, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorTuya Shilagard MS
Center for Biomedical Engineering, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorGracie Vargas PhD
Center for Biomedical Engineering, UTMB Health Cancer Center, Galveston, Texas
Department of Neuroscience and Cell Biology, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorCorresponding Author
Vicente A. Resto MD, PhD
Department of Otolaryngology, UTMB Health Cancer Center, Galveston, Texas
UTMB Health Cancer Center, Galveston, Texas
Department of Biochemistry and Molecular Biology, UTMB Health Cancer Center, Galveston, Texas
Corresponding author: V. A. Resto, Department of Otolaryngology, UTMB Health, 301 University Blvd., Galveston, TX 77555-0521. E-mail: [email protected]Search for more papers by this authorPeter Szaniszlo MD, PhD
Department of Otolaryngology, UTMB Health Cancer Center, Galveston, Texas
UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorSusan M. Fennewald PhD
Department of Otolaryngology, UTMB Health Cancer Center, Galveston, Texas
UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorSuimin Qiu MD, PhD
UTMB Health Cancer Center, Galveston, Texas
Department of Pathology, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorCarla Kantara PhD
UTMB Health Cancer Center, Galveston, Texas
Department of Neuroscience and Cell Biology, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorTuya Shilagard MS
Center for Biomedical Engineering, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorGracie Vargas PhD
Center for Biomedical Engineering, UTMB Health Cancer Center, Galveston, Texas
Department of Neuroscience and Cell Biology, UTMB Health Cancer Center, Galveston, Texas
Search for more papers by this authorCorresponding Author
Vicente A. Resto MD, PhD
Department of Otolaryngology, UTMB Health Cancer Center, Galveston, Texas
UTMB Health Cancer Center, Galveston, Texas
Department of Biochemistry and Molecular Biology, UTMB Health Cancer Center, Galveston, Texas
Corresponding author: V. A. Resto, Department of Otolaryngology, UTMB Health, 301 University Blvd., Galveston, TX 77555-0521. E-mail: [email protected]Search for more papers by this authorThis work was presented in part at the 8th International Conference on Head and Neck Cancer, American Head and Neck Society, Toronto, Ontario, Canada, July 21–25, 2012.
Abstract
Background
The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.
Methods
HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.
Results
Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.
Conclusion
This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology. © 2014 Wiley Periodicals, Inc. Head Neck 36: 1638–1647, 2014
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