Isolation and genomic characterization of stem cells in head and neck cancer
Corresponding Author
George D. Wilson PhD
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan. E-mail: [email protected]Search for more papers by this authorBrian Marples PhD
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorSandra Galoforo MS
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorTimothy J. Geddes MS
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorBryan J. Thibodeau PhD
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorReidar Grénman MD, PhD
Department of Otorhinolaryngology–Head and Neck Surgery, University of Turku and Turku University Hospital, Turku, Finland
Search for more papers by this authorJan Akervall MD, PhD
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Department of Otolaryngology, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorCorresponding Author
George D. Wilson PhD
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan. E-mail: [email protected]Search for more papers by this authorBrian Marples PhD
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorSandra Galoforo MS
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorTimothy J. Geddes MS
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorBryan J. Thibodeau PhD
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorReidar Grénman MD, PhD
Department of Otorhinolaryngology–Head and Neck Surgery, University of Turku and Turku University Hospital, Turku, Finland
Search for more papers by this authorJan Akervall MD, PhD
Beaumont BioBank, William Beaumont Hospital, Royal Oak, Michigan
Department of Otolaryngology, William Beaumont Hospital, Royal Oak, Michigan
Search for more papers by this authorThis research was presented at: ASCO-NCI-EOTRC Molecular Markers in Cancer, Hollywood, Florida, October 2010 and ASTRO, San Diego, California, November 2010.
Abstract
Background
This study investigated the use of 3 different established cell-sorting strategies to isolate and characterize stem cells from head and neck cancer cell lines.
Methods
Five low-passage cell lines were subjected to cell sorting based on Hoechst side population, Aldefluor, and CD44 expression. Isolated cell populations were studied for gene expression, radiosensitivity, and chemosensitivity to cisplatin and paclitaxel.
Results
Each sorting method identified a different set of genes associated with different gene ontology categories, with mitosis being the only common category. CD44-associated gene changes were almost exclusively associated with cell cycle and in particular mitosis. There were no significant differences in radiosensitivity or cisplatin sensitivity of stem or non–stem cells, but CD44-isolated stem cells were more resistant to paclitaxel.
Conclusions
This study suggested that CD44 may be the most promising cell-sorting strategy to isolate and investigate the impact of stem cells in head and neck squamous cell cancer (HNSCC). © 2012 Wiley Periodicals, Inc. Head Neck, 35: 1573–1582, 2013
Supporting Information
Additional Supporting Information may be found in the online version of this article.
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| hed23184-sup-0001-SuppFig1.tif349.8 KB | Supporting Information Figure 1. |
| hed23184-sup-0002-SuppFig2.tif106.2 KB | Supporting Information Figure 2. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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