UM-SCC-104: A New human papillomavirus-16–positive cancer stem cell–containing head and neck squamous cell carcinoma cell line†
Alice L. Tang MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorSamantha J. Hauff MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorJohn H. Owen MS
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorMartin P. Graham BS
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorMichael J. Czerwinski
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorJung Je Park MD, PhD
Department of Otolaryngology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jin Ju, South Korea
Search for more papers by this authorHeather Walline MA
Sequenom Molecular Medicine Laboratory, Ann Arbor, Michigan
Pre-Doctoral Candidate Graduate Program in Cancer Biology, The University of Michigan School of Medicine, Ann Arbor, Michigan
Search for more papers by this authorSilvana Papagerakis MD, PhD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorJay Stoerker PhD
Sequenom Molecular Medicine Laboratory, Ann Arbor, Michigan
Vice President and Laboratory Director, aMDX Laboratory Sciences, Farmington Hills, Michigan
Search for more papers by this authorJonathan B. McHugh MD
Department of Pathology, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorDouglas B. Chepeha MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorCarol R. Bradford MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorCorresponding Author
Thomas E. Carey PhD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Thomas E. Carey and Mark E. Prince contributed equally to this work.
Department of Otolaryngology – Head & Neck Surgery, University of Michigan, Ann Arbor, MichiganSearch for more papers by this authorMark E. Prince MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Thomas E. Carey and Mark E. Prince contributed equally to this work.
Search for more papers by this authorAlice L. Tang MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorSamantha J. Hauff MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorJohn H. Owen MS
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorMartin P. Graham BS
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorMichael J. Czerwinski
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorJung Je Park MD, PhD
Department of Otolaryngology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jin Ju, South Korea
Search for more papers by this authorHeather Walline MA
Sequenom Molecular Medicine Laboratory, Ann Arbor, Michigan
Pre-Doctoral Candidate Graduate Program in Cancer Biology, The University of Michigan School of Medicine, Ann Arbor, Michigan
Search for more papers by this authorSilvana Papagerakis MD, PhD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorJay Stoerker PhD
Sequenom Molecular Medicine Laboratory, Ann Arbor, Michigan
Vice President and Laboratory Director, aMDX Laboratory Sciences, Farmington Hills, Michigan
Search for more papers by this authorJonathan B. McHugh MD
Department of Pathology, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorDouglas B. Chepeha MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorCarol R. Bradford MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Search for more papers by this authorCorresponding Author
Thomas E. Carey PhD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Thomas E. Carey and Mark E. Prince contributed equally to this work.
Department of Otolaryngology – Head & Neck Surgery, University of Michigan, Ann Arbor, MichiganSearch for more papers by this authorMark E. Prince MD
Department of Otolaryngology—Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan
Thomas E. Carey and Mark E. Prince contributed equally to this work.
Search for more papers by this authorThis work was presented at the American Head and Neck Society 2010 Research Workshop Meeting in Arlington, Virginia, October 28–30, 2010.
Dr. Stoerker and Ms. Walline were employees of Sequenom when this work was performed. Sequenom had a proprietary interest in the PCR-MassArray Assay, an intellectual property of the university of Michigan. Dr. Stoerker had a financial interest in Sequenom at that time.
Abstract
Background
Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan-squamous cell carcinoma-104 (UM-SCC-104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs).
Methods
Tumor cells were tested for biomarker expression by immunohistology, and the presence of HPV was assessed by several methods.
Results
UM-SCC-104 has a unique genotype, contains HPV-16, and expresses E6/E7. Inoculation of aldehyde dehydrogenase (ALDH)(+) and ALDH(−) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, whereas ALDH(−) cells did not produce tumors.
Conclusion
UM-SCC-104, a new HPV-16, CSC-containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of epidermal growth factor receptor (EGFR) and a functionally significant proportion of ALDH(+) CSCs. © 2011 Wiley Periodicals, Inc. Head Neck, 2011
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