Murine model of neuromuscular electrical stimulation on squamous cell carcinoma: Potential implications for dysphagia therapy
Gary Linkov BS
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorRyan C. Branski PhD
Department of Otolaryngology, New York University Medical Center, New York, New York
Search for more papers by this authorMilan Amin MD
Department of Otolaryngology, New York University Medical Center, New York, New York
Search for more papers by this authorNatalya Chernichenko MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorChun-Hao Chen MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorGad Alon PhD
Physical Therapy and Rehabilitation Sciences, University of Maryland, School of Medicine, Baltimore, Maryland
Search for more papers by this authorSusan Langmore PhD
Department of Otolaryngology–Head and Neck Surgery, Boston University Medical Center; Speech Language Hearing Department, Boston University, Boston, Massachusetts
Search for more papers by this authorRichard J. Wong MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorCorresponding Author
Dennis H. Kraus MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New YorkSearch for more papers by this authorGary Linkov BS
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorRyan C. Branski PhD
Department of Otolaryngology, New York University Medical Center, New York, New York
Search for more papers by this authorMilan Amin MD
Department of Otolaryngology, New York University Medical Center, New York, New York
Search for more papers by this authorNatalya Chernichenko MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorChun-Hao Chen MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorGad Alon PhD
Physical Therapy and Rehabilitation Sciences, University of Maryland, School of Medicine, Baltimore, Maryland
Search for more papers by this authorSusan Langmore PhD
Department of Otolaryngology–Head and Neck Surgery, Boston University Medical Center; Speech Language Hearing Department, Boston University, Boston, Massachusetts
Search for more papers by this authorRichard J. Wong MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Search for more papers by this authorCorresponding Author
Dennis H. Kraus MD
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York
Head and Neck Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New YorkSearch for more papers by this authorAbstract
Background
Dysphagia is a potential consequence of treatment for head and neck cancer. Neuromuscular electrical stimulation (NMES) has evolved as a treatment option, with the goal of improved swallow function in patients with chronic dysphagia. However, the effects of NMES on tumorigenicity are unknown and often confound the initiation of this therapy, potentially limiting its efficacy in treating patients with head and neck cancer.
Methods
Squamous cell carcinoma was grown in the flank of athymic, nude mice. Mice were randomized into treatment and control groups; the experimental group received daily NMES directly to the flank for 8 days.
Results
Tumor volumes, recorded on days 0, 3, 7, and 10, demonstrated no significant differences between groups on each day of measurement. Immunohistochemical analysis of apoptosis, proliferation, and vascularization also failed to demonstrate statistically significant differences between treated and untreated groups.
Conclusions
NMES does not promote the growth of underlying tumor in our model. These data may provide preliminary evidence that applying electrical stimulation over the muscles of the anterior neck does not increase the risk of tumorigenicity. Early initiation of NMES in this challenging population may be feasible from an oncologic standpoint. © 2011 Wiley Periodicals, Inc. Head Neck, 2011
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