Combined haploid and insertional mutation screen in the zebrafish
Elizabeth Wiellette
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Search for more papers by this authorYevgenya Grinblat
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Y. Grinblat and M. Austen contributed equally to this work.
Search for more papers by this authorMatthias Austen
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Y. Grinblat and M. Austen contributed equally to this work.
Search for more papers by this authorEstelle Hirsinger
Institute of Neuroscience, University of Oregon, Eugene, Oregon
Search for more papers by this authorAdam Amsterdam
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts
Search for more papers by this authorCharline Walker
Institute of Neuroscience, University of Oregon, Eugene, Oregon
Search for more papers by this authorMonte Westerfield
Institute of Neuroscience, University of Oregon, Eugene, Oregon
Search for more papers by this authorCorresponding Author
Hazel Sive
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Massachusetts Institute of Technology, Cambridge, Massachusetts
Massachusetts Institute of Technology, Cambridge, MA 02139Search for more papers by this authorElizabeth Wiellette
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Search for more papers by this authorYevgenya Grinblat
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Y. Grinblat and M. Austen contributed equally to this work.
Search for more papers by this authorMatthias Austen
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Y. Grinblat and M. Austen contributed equally to this work.
Search for more papers by this authorEstelle Hirsinger
Institute of Neuroscience, University of Oregon, Eugene, Oregon
Search for more papers by this authorAdam Amsterdam
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts
Search for more papers by this authorCharline Walker
Institute of Neuroscience, University of Oregon, Eugene, Oregon
Search for more papers by this authorMonte Westerfield
Institute of Neuroscience, University of Oregon, Eugene, Oregon
Search for more papers by this authorCorresponding Author
Hazel Sive
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
Massachusetts Institute of Technology, Cambridge, Massachusetts
Massachusetts Institute of Technology, Cambridge, MA 02139Search for more papers by this authorAbstract
To identify genes required for development of the brain and somites, we performed a pilot screen of gynogenetic haploid zebrafish embryos produced from mothers mutagenized by viral insertion. We describe an efficient method to identify new mutations and the affected gene. In addition, we report the results of a small-scale screen that identified five genes required for brain development, including novel alleles of nagie oko, pou5f1, ribosomal protein L36, and n-cadherin, as well as a novel allele of the laminin g1 gene that is required for normal skeletal muscle fiber organization and somite patterning. genesis 40:231–240, 2004. © 2004 Wiley-Liss, Inc.
LITERATURE CITED
- Akimenko MA, Ekker M, Wegner J, Lin W, Westerfield M. 1994. Combinatorial expression of three zebrafish genes related to distal-less: part of a homeobox gene code for the head. J Neurosci 14: 3475–3486.
-
Alexander J,
Stainier DY,
Yelon D.
1998.
Screening mosaic F1 females for mutations affecting zebrafish heart induction and patterning.
Dev Genet
22:
288–299.
10.1002/(SICI)1520-6408(1998)22:3<288::AID-DVG10>3.0.CO;2-2 CAS PubMed Web of Science® Google Scholar
- Amsterdam A. 2003. Insertional mutagenesis in zebrafish. Dev Dyn 228: 523–534.
- Amsterdam A, Hopkins N. 1999. Retrovirus-mediated insertional mutagenesis in zebrafish. Methods Cell Biol 60: 87–98.
- Amsterdam A, Burgess S, Golling G, Chen W, Sun Z, Townsend K, Farrington S, Haldi M, Hopkins N. 1999. A large-scale insertional mutagenesis screen in zebrafish. Genes Dev 13: 2713–2724.
- Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N. 2004a. 315 Genes essential for early zebrafish development. Proc Natl Acad Sci USA 101: 12792–12797.
- Amsterdam A, Sadler KC, Lai K, Farrington S, Bronson RT, Lees JA, Hopkins N. 2004b. Many ribosomal protein genes are cancer genes in zebrafish. PLoS Biol 2: E139.
- Bader D, Masaki T, Fischman DA. 1982. Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro. J Cell Biol 95: 763–770.
- Belting HG, Hauptmann G, Meyer D, Abdelilah-Seyfried S, Chitnis A, Eschbach C, Soll I, Thisse C, Thisse B, Artinger KB, Lunde K, Driever W. 2001. Spiel ohne grenzen/pou2 is required during establishment of the zebrafish midbrain-hindbrain boundary organizer. Development 128: 4165–4176.
- Burgess S, Reim G, Chen W, Hopkins N, Brand M. 2002. The zebrafish spiel-ohne-grenzen (spg) gene encodes the POU domain protein Pou2 related to mammalian Oct4 and is essential for formation of the midbrain and hindbrain, and for pre-gastrula morphogenesis. Development 129: 905–916.
- Devoto SH, Melancon E, Eisen JS, Westerfield M. 1996. Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation. Development 122: 3371–3380.
- Driever W, Solnica-Krezel L, Schier AF, Neuhauss SC, Malicki J, Stemple DL, Stainier DY, Zwartkruis F, Abdelilah S, Rangini Z, Belak J, Boggs C. 1996. A genetic screen for mutations affecting embryogenesis in zebrafish. Development 123: 37–46.
- Golling G, Amsterdam A, Sun Z, Antonelli M, Maldonado E, Chen W, Burgess S, Haldi M, Artzt K, Farrington S, Lin SY, Nissen RM, Hopkins N. 2002. Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development. Nat Genet 31: 135–140.
- Grunwald DJ, Kimmel CB, Westerfield M, Walker C, Streisinger G. 1988. A neural degeneration mutation that spares primary neurons in the zebrafish. Dev Biol 126: 115–128.
- Haffter P, Granato M, Brand M, Mullins MC, Hammerschmidt M, Kane DA, Odenthal J, van Eeden FJ, Jiang YJ, Heisenberg CP, Kelsh RN, Furutani-Seiki M, Vogelsang E, Beuchle D, Schach U, Fabian C, Nusslein-Volhard C. 1996. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development 123: 1–36.
- Halpern ME, Ho RK, Walker C, Kimmel CB. 1993. Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation. Cell 75: 99–111.
- Hug B, Walter V, Grunwald DJ. 1997. tbx6, a Brachyury-related gene expressed by ventral mesendodermal precursors in the zebrafish embryo. Dev Biol 183: 61–73.
- Jensen AM, Westerfield M. 2004. Zebrafish mosaic eyes is a novel FERM protein required for retinal lamination and retinal pigmented epithelial tight junction formation. Curr Biol 14: 711–717.
- Jiang YJ, Brand M, Heisenberg CP, Beuchle D, Furutani-Seiki M, Kelsh RN, Warga RM, Granato M, Haffter P, Hammerschmidt M, Kane DA, Mullins MC, Odenthal J, van Eeden FJ, Nusslein-Volhard C. 1996. Mutations affecting neurogenesis and brain morphology in the zebrafish, Danio rerio. Development 123: 205–216.
- Krauss S, Johansen T, Korzh V, Fjose A. 1991. Expression of the zebrafish paired box gene pax[zf-b] during early neurogenesis. Development 113: 1193–1206.
- Krauss S, Concordet JP, Ingham PW. 1993. A functionally conserved homolog of the Drosophila segment polarity gene hh is expressed in tissues with polarizing activity in zebrafish embryos. Cell 75: 1431–1444.
- Lele Z, Folchert A, Concha M, Rauch GJ, Geisler R, Rosa F, Wilson SW, Hammerschmidt M, Bally-Cuif L. 2002. Parachute/n-cadherin is required for morphogenesis and maintained integrity of the zebrafish neural tube. Development 129: 3281–3294.
- Loosli F, Staub W, Finger-Baier KC, Ober EA, Verkade H, Wittbrodt J, Baier H. 2003. Loss of eyes in zebrafish caused by mutation of chokh/rx3. EMBO Rep 4: 894–899.
- Miller JB, Crow MT, Stockdale FE. 1985. Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures. J Cell Biol 101: 1643–1650.
- Moens CB, Yan YL, Appel B, Force AG, Kimmel CB. 1996. Valentino: a zebrafish gene required for normal hindbrain segmentation. Development 122: 3981–3990.
- Mullins MC. 2002. Building-blocks of embryogenesis. Nat Genet 31: 125–126.
- Oxtoby E, Jowett T. 1993. Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development. Nucleic Acids Res 21: 1087–1095.
- Parsons MJ, Pollard SM, Saude L, Feldman B, Coutinho P, Hirst EM, Stemple DL. 2002. Zebrafish mutants identify an essential role for laminins in notochord formation. Development 129: 3137–3146.
- Patton EE, Zon LI. 2001. The art and design of genetic screens: zebrafish. Nat Rev Genet 2: 956–966.
- Sagerstrom CG, Grinbalt Y, Sive H. 1996. Anteroposterior patterning in the zebrafish, Danio rerio: an explant assay reveals inductive and suppressive cell interactions. Development 122: 1873–1883.
- Walker C. 1999. Haploid screens and gamma-ray mutagenesis. In: W Detrich, M Westerfield, LI Zon, editors. The zebrafish: genetics and genomics. San Diego: Academic Press. p 44–70.
- Wei X, Malicki J. 2002. Nagie oko, encoding a MAGUK-family protein, is essential for cellular patterning of the retina. Nat Genet 31: 150–157.
- Westerfield M. 1987. Substrate interactions affecting motor growth cone guidance during development and regeneration. J Exp Biol 132: 161–175.
- Westerfield M. 2000. The zebrafish book, 3rd ed. Eugene: University of Oregon Press.
