A heat shock-like protein from the human malaria parasite plasmodium falciparum induces autoantibodies
Corresponding Author
Denise Mattei
Experimental Parasitology Unit, Institut Pasteur, Paris
Institut Pasteur, 25–28, rue du Docteur Roux, F-75724 Paris CEDEX 15, FranceSearch for more papers by this authorArtur Scherf
Experimental Parasitology Unit, Institut Pasteur, Paris
Recipient of an EMBO fellowship.
Search for more papers by this authorOliver Bensaude
Molecular Biology of Stress, Institut Pasteur, Paris
Search for more papers by this authorLuiz Pereira Da Silva
Experimental Parasitology Unit, Institut Pasteur, Paris
Search for more papers by this authorCorresponding Author
Denise Mattei
Experimental Parasitology Unit, Institut Pasteur, Paris
Institut Pasteur, 25–28, rue du Docteur Roux, F-75724 Paris CEDEX 15, FranceSearch for more papers by this authorArtur Scherf
Experimental Parasitology Unit, Institut Pasteur, Paris
Recipient of an EMBO fellowship.
Search for more papers by this authorOliver Bensaude
Molecular Biology of Stress, Institut Pasteur, Paris
Search for more papers by this authorLuiz Pereira Da Silva
Experimental Parasitology Unit, Institut Pasteur, Paris
Search for more papers by this authorAbstract
The humoral immune response to a 72-kDa heat shock-like protein of Plasmodium falciparum has been analyzed using mouse monoclonal antibodies (mAb) and human immune sera. Three regions of the molecule containing B cell epitopes were identified by screening a sublibrary encoding the COOH-terminal half of the antigen with the mAb. One B cell epitope mapped to a region poorly conserved between the parasite 72-kDa polypeptide and mammalian heat-shock proteins (Hsp 70). Another mAb, G10C9, reacted with an amino acid region that has a high degree of homology with mouse (87.5%) and human (81.2%) Hsp 70. Both mouse and human cells were recognized by this mAb when analyzed by indirect immunofluorescence and by two-dimensional immunoblots. Sera from humans infected with malaria also recognized the human Hsp 70. Thus, our results indicate that autoantibodies directed against host Hsp 70 can be induced by the homologous parasite protein.
References
- 1 Cohen, S., McGregor, I. A. and Carrington, S. P., Nature 1961. 192: 733.
- 2 Rosenberg, E. B., Strickland, G. T., Yang, S.-L. and Whalen, G. E., Am. J. Trop. Med. Hyg. 1973. 22: 146.
- 3 Zouali, M., Druilhe, P., Gentilini, M. and Eyquem, A., Clin. Exp. Immunol. 1982. 50: 83.
- 4 Berzins, K., Wahlgren, M. and Perlmann, P., Clin. Exp. Immunol. 1983. 54: 313.
- 5 Wells, R. A., Pavanand, K., Zolyomi, S., Permpanich, B. and MacDermott, R. P., Clin. Exp. Immunol. 1980. 39: 663.
- 6 Sharper, A. G., Kaplan, M. H., Mody, N. J. and McIntyre, P. A., Lancet 1968. 1: 1342.
- 7 Greenwood, B. M., Herrick, E. M. and Holborow, E. J., Clin. Exp. Immunol. 1970. 7: 75.
- 8 Adu, D. D., Willians, G., Quakyi, I. A., Voller, A., Anim-Addo, Y., Bruce-Tagoe, A. A., Johnson, G. D. and Holborow, E. J., Clin. Exp. Immunol. 1982. 49: 310.
- 9 McLaughlin, G. L., Benedick, M. J. and Campbell, G. H., Am. J. Trop. Med. Hyg. 1987. 37: 258.
- 10 Cheung, A., Leban, J., Shaw, A. R., Merkli, B., Stocker, J., Chizzolini, C., Sander, C. and Perrin, L. H., Proc. Natl. Acad. Sci. USA 1986. 83: 8328.
- 11 Certa, U., Ghersa, P., Döbeli, H., Matile, H., Kocher, H. C., Shrivastava, I. K., Shaw, A. R. and Perrin, L., Science 1988. 240: 1036.
- 12 Kaslow, D. C., Quakyi, I. A., Syin, C., Raum, M. G., Keister, D. B., Coligan, J. E., McCutchan, T. F. and Miller, L. H., Nature 1988. 333: 74.
- 13 Dubois, P., Dedet, J. P., Fandeur, T., Roussilhon, C., Jendoubi, M., Pauillac, S., Mercereau-Puijalon, O. and Pereira da Silva, L., Proc. Natl. Acad. Sci. USA 1984. 81: 229.
- 14 Bianco, A. E., Favaloro, J. M., Burkot, T. R., Culvenor, J. G., Crewther, P. E., Brown, G. V., Anders, R. F., Coppel, R. L., and Kemp, D. J., Proc. Natl. Acad. Sci. USA 1986. 83: 8713.
- 15 Ardeshir, F., Flint, J. E., Richman, S. J. and Reese, R. T., EMBO J. 1987. 6: 493.
- 16 Yang, Y.-F., Tan-ariya, P., Sharma, Y. D. and Kilejian, A., Mol. Biochem. Parasitol. 1987. 26: 61.
- 17 Mattei, D., Ozaki, L. S. and Pereira da Silva, L., Nucleic Acids Res. 1988. 16: 5204.
- 18 Hedstrom, R., Culpepper, J., Harrison, R. A., Agabian, N. and Newport, G., J. Exp. Med. 1987. 165: 1430.
- 19 Scallon, B. J., Bogitsh, B. J. and Carter, C. E., Mol. Biochem. Parasitol. 1987. 24: 237.
- 20 Hedstrom, R., Culpepper, J., Schinski, V., Agabian, N. and Newport, G., Mol. Biochem. Parasitol. 1988. 29: 275.
- 21 Young, D., Lathigra, R., Hendrix, R., Sweetser, D. and Young, R. A., Proc. Natl. Acad. Sci. USA 1988. 85: 4267.
- 22 Trager, W. and Jensen, J. B., Science 1976. 193: 673.
- 23 Bensaude, O. and Morange, M., EMBO J. 1983. 2: 173.
- 24 Ozaki, L. S., Jendoubi, M., Mattei, D., Braun-Breton, C., Jouin, H., Blisnick, T. and Pereira da Silva, L., in N. Agabian, H. Goodman and N. Nogueira (Eds.), Molecular Strategies of Parasitic Invasion. Alan R. Liss, Inc. New York 1987, p. 683.
- 25 Blisnick, T., Lema, F., Mazie, J. C. and Pereira da Silva, L., Exp. Parasitol. 1988. 67: 247.
- 26 Mattei, D., Langsley, G., Braun-Breton, C., Guillotte, M., Dubremetz, J.-F. and Mercereau-Puijalon, O., Mol. Biochem. Parasitol. 1988. 27: 171.
- 27 Messing, J. and Vieira, J., Gene 1982. 19: 269.
- 28 Sanger, F., Nicklen, S. and Coulson, A. R., Proc. Natl. Acad. Sci. USA 1977. 74: 5463.
- 29 Biggin, M. D., Gibson, T. J. and Hong, G. F., Proc. Natl. Acad. Sci. USA 1983. 80: 3963.
- 30 Chen, E. J. and Seeburg, P. H., DNA 1985. 4: 165.
- 31 Laemmli, U. K., Nature 1970. 227: 680.
- 32 Goding, J. W. and Handman, E., in C. M. Morel (Ed.), Genes and Antigens of Parasites. Rio de Janeiro, Brasil 1984, p. 383.
- 33 Ozaki, L. S., Mattei, D., Jendoudi, M., Druilhe, P., Blisnick, T., Guillotte, M., Puijalon, O. and Pereira da Silva, L., J. Immunol. Methods 1986. 89: 213.
- 34 O'Farrell, P. H., J. Biol. Chem. 1975. 250: 4007.
- 35 Milarski, K. L. and Morimoto, R. I., Proc. Natl. Acad. Sci. USA 1986. 83: 9517.
- 36 Dworniczak, B. and Mirault, M.-E., Nucleic Acids Res. 1987. 15: 5181.
- 37 Hunt, C. and Morimoto, R. I., Proc. Natl. Acad. Sci USA 1985. 82: 6455.
- 38 Lowe, D. G. and Moran, L. A., J. Biol. Chem. 1986. 261: 2102.
- 39 Haas, I. and Meo, T., Proc. Natl. Acad. Sci. USA 1988. 85: 2250.
- 40 Anderson, N. L., Giometti, C. S., Gemmell, M. A., Nance, S. L. and Anderson, N. G., Clin. Chem. 1982. 28: 1084.
- 41 Lindquist, S., Annu. Rev. Biochem. 1986. 55: 1151.
- 42 Chou, P. Y. and Fasman, G. D., Biochemistry 1974. 13: 211.
- 43 Margalit, H., Spouge, J. L., Cornette, J. L., Cease, K. B., De Lisi, C. and Berzofsky, J. A., J. Immunol. 1987. 138: 2213.
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