A new V gene expressed in lambda-2 light chains of the mouse
Renate Dildrop
Institute for Genetics, University of Cologne, Cologne
Search for more papers by this authorAngela Gause
Institute for Genetics, University of Cologne, Cologne
Recipient of a long-term fellowship from the Deutsche Forschungsgemeinschaft.
Search for more papers by this authorWerner Müller
Institute for Genetics, University of Cologne, Cologne
Search for more papers by this authorCorresponding Author
Klaus Rajewsky
Institute for Genetics, University of Cologne, Cologne
Institut für Genetik, Weyertal 121, D-5000 Köln 41, FRGSearch for more papers by this authorRenate Dildrop
Institute for Genetics, University of Cologne, Cologne
Search for more papers by this authorAngela Gause
Institute for Genetics, University of Cologne, Cologne
Recipient of a long-term fellowship from the Deutsche Forschungsgemeinschaft.
Search for more papers by this authorWerner Müller
Institute for Genetics, University of Cologne, Cologne
Search for more papers by this authorCorresponding Author
Klaus Rajewsky
Institute for Genetics, University of Cologne, Cologne
Institut für Genetik, Weyertal 121, D-5000 Köln 41, FRGSearch for more papers by this authorAbstract
We have partially sequenced the light chain variable regions expressed in three IgM-producing hybridomas generated from newborn mice or from manipulated animals suppressed for IgM production. In these lines a new V gene (V-lambda-X), exhibiting less than 60% homology to any known lambda or kappa V gene, is rearranged to J-lambda-2. The light chains produced by these cells contain the lambda-2 constant domain, but are not recognized by goat antisera raised against conventional mouse lambda light chains.
References
- 1 Weigert, M. and Riblet, R., Cold Spring Harbor Symp. Quant. Biol. 1976. 41: 837.
- 2 Dugan, E. S., Bradshaw, R. A., Simms, E. S. and Eisen, H. N., Biochemistry 1973. 12: 5400.
- 3 Azuma, T., Steiner, L. A. and Eisen, H., Proc. Natl. Acad. Sci. USA 1981. 78: 569.
- 4 Miller, J., Bothwell, A. and Storb, U., Proc. Natl. Acad. Sci. USA 1981. 78: 3829.
- 5 Miller, J., Selsing, E. and Storb, U., Nature 1982. 295: 428.
- 6 Blomberg, B., Traunecker, A., Eisen, H. and Tonegawa, S., Proc. Natl. Acad. Sci. USA 1981. 78: 3765.
- 7 Bernard, O., Hozumi, N. and Tonegawa, S., Cell 1978. 15: 1133.
- 8 Tonegawa, S., Maxam, A. M., Tizard, R., Bernard, O. and Gilbert, W., Proc. Natl. Acad. Sci. USA 1978. 75: 1485.
- 9 Blomberg, B. and Tonegawa, S., Proc. Natl. Acad. Sci. USA 1982. 79: 530.
- 10 Selsing, E., Miller, J., Wilson, R. and Storb, U., Proc. Natl. Acad. Sci. USA 1982. 79: 4681.
- 11 Click, R. E., Benck, L. and Alter, B. J., Cell. Immunol. 1972. 3: 264.
- 12 Kearney, J. F., Radbruch, A., Liesegang, B. and Rajewsky, K., J. Immunol. 1979. 123: 1548.
- 13 Nishikawa, S., Sasaki, Y., Kina, T., Amagi, T. and Katsura, Y., Immunogenetics 1986. 23: 137.
- 14 Laemmli, U. K., Nature 1970. 227: 680.
- 15 Siekevitz, M., Huang, S. Y. and Gefter, M. L., Eur. J. Immunol. 1983. 13: 123.
- 16 Cumano, A. and Rajewsky, K., Eur. J. Immunol. 1985. 15: 512.
- 17 Schreier, P. H., Quester, S. and Bothwell, A. L. M., Nucleic Acids Res. 1986. 14: 2381.
- 18 Boersch-Supan, M. E., Agarwal, S., White-Scharf, M. E. and Imanishi-Kari, T., J. Exp. Med. 1985. 161: 1272.
- 19 Siekevitz, M., Kocks, C., Rajewsky, K. and Dildrop, R., Cell 1987, in press.
- 20 Durdik, J., Moore, M. W. and Selsing, E., Nature 1984. 307: 749.
- 21 Moore, M. W., Durdik, J., Persiani, D. M. and Selsing, E., Proc. Natl. Acad. Sci. USA 1985. 82: 6211.
- 22 Picard, D. and Schaffner, W., Proc. Natl. Acad. Sci. USA 1983. 80: 417.
- 23 Bothwell, A. L. M., Paskind, M., Schwartz, R., Sonnenshein, G., Gefter, M. and Baltimore, D., Nature 1981. 290: 65.
- 24 Reth, M., Hämmerling, G. J. and Rajewsky, K., Eur. J. Immunol. 1978. 8: 393.
- 25 Reth, M., Gehrmann, P., Petrac, E. and Wiese, P., Nature 1986. 322: 840.
- 26 Kleinfield, R., Hardy, R., Tarlinton, D., Dangl, J., Herzenberg, L. A. and Weigert, M., Nature 1986. 322: 843.
- 27 Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Müller, M. and Perry, H., Sequences of Proteins of Immunological Interest, U.S. Department of Health and Human Services, Public Health Service, Bethesda, MD 1983.
Citing Literature
