Effect of anti-γ3 antibodies on immunoglobulin isotype expression in lipopolysaccharide-stimulated cultures of mouse spleen cells
Corresponding Author
Carol F. Webb
The Cellular Immunobiology Unit of the Tumor Institute, Birmingham
224 Tumor Institute, University of Alabama in Birmingham, University Station, Birmingham, AL 35294, USASearch for more papers by this authorWilliam E. Gathings
Departments of Pediatrics and Microbiology, Birmingham
Search for more papers by this authorMax D. Cooper
The Comprehensive Cancer Center University of Alabama in Birmingham, Birmingham
Search for more papers by this authorCorresponding Author
Carol F. Webb
The Cellular Immunobiology Unit of the Tumor Institute, Birmingham
224 Tumor Institute, University of Alabama in Birmingham, University Station, Birmingham, AL 35294, USASearch for more papers by this authorWilliam E. Gathings
Departments of Pediatrics and Microbiology, Birmingham
Search for more papers by this authorMax D. Cooper
The Comprehensive Cancer Center University of Alabama in Birmingham, Birmingham
Search for more papers by this authorAbstract
To test the hypothesis that γ3 is the pivotal isotype for sequential heavy chain switching from μ to each of the γ isotypes, we have compared the effects of anti-γ3 and anti-μ antibodies on the expression of immunoglobulin isotypes in lipopolysaccharide (LPS)-stimulated cultures of mouse spleen cells. IgM-, IgG1-, IgG2b- and IgG2a-containing plasma cells were enumerated by immunofluorescence and secreted immunoglobulins were measured by radioimmunoassay. Although anti-γ3 and anti-μ, were equally effective in inhibiting the LPS-induced differentiation of IgG3 plasma cells, anti-γ3 had no effect on the differentiation of IgM, IgG1, IgG2b, or IgG2a plasma cells. These results support a direct mechanism of heavy chain immunoglobulin switching.
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