Detection of Epstein-Barr virus (EBV) in the oral mucosa of renal transplant patients
Paulo H. Braz-Silva D.D.S.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorKarem L. Ortega D.D.S., Ph.D.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorNathalie P. Rezende D.D.S., M.D.S.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorFabio D. Nunes D.D.S., Ph.D.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
Marina H.C.G. Magalhães, D.D.S., Ph.D.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Department of Oral Pathology, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227 Cidade Universitária, 05508-900 São Paulo, SP, BrazilSearch for more papers by this authorPaulo H. Braz-Silva D.D.S.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorKarem L. Ortega D.D.S., Ph.D.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorNathalie P. Rezende D.D.S., M.D.S.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorFabio D. Nunes D.D.S., Ph.D.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
Marina H.C.G. Magalhães, D.D.S., Ph.D.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil
Department of Oral Pathology, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227 Cidade Universitária, 05508-900 São Paulo, SP, BrazilSearch for more papers by this authorAbstract
The purpose of this study was to determine the prevalence of Epstein-Barr virus (EBV)-DNA in the oral mucosa of renal transplant patients and observe the efficacy of mouth rinses with phosphate-buffered saline (PBS) to eliminate EBV present in the saliva. Lingual, gingival, and buccal cytobrushings were obtained from normal oral mucosa of 10 renal transplant patients and 10 normal subjects, and were examined through polymerase chain reaction (PCR), before and after rinses with PBS. EBV-DNA was detected in 86.6% of renal transplant recipients and in 46.6% of healthy subjects. No significant difference was observed between oral scrapes obtained before and after rinses with PBS with regard to detection of EBV-DNA. Our results suggest that the use of PCR to detect the presence of EBV-DNA in oral mucosa in the absence of specific lesions gives rise to the problem of identifying the viral replication sites. In addition, PBS was not effective at minimizing contamination by saliva. Diagn. Cytopathol. 2006;34:24–28. © 2005 Wiley-Liss, Inc.
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