Migration of Dictyostelium slugs: Anterior-like cells may provide the motive force for the prespore zone
Corresponding Author
Jean-Paul Rieu
Laboratoire de Physique de la Matière Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Jean-Paul Rieu, Laboratoire de Physique de la Matib̀eère Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Robert R. Kay, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom
Search for more papers by this authorTamao Saito
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
Search for more papers by this authorHéléne Delanoë-Ayari
Laboratoire de Physique de la Matière Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Search for more papers by this authorYasuji Sawada
Tohoku Institute of Technology, 35-1 Yagiyama-Kasumi, Taihaku, 983, Sendai, Japan
Search for more papers by this authorCorresponding Author
Robert R. Kay
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom
Jean-Paul Rieu, Laboratoire de Physique de la Matib̀eère Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Robert R. Kay, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom
Search for more papers by this authorCorresponding Author
Jean-Paul Rieu
Laboratoire de Physique de la Matière Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Jean-Paul Rieu, Laboratoire de Physique de la Matib̀eère Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Robert R. Kay, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom
Search for more papers by this authorTamao Saito
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
Search for more papers by this authorHéléne Delanoë-Ayari
Laboratoire de Physique de la Matière Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Search for more papers by this authorYasuji Sawada
Tohoku Institute of Technology, 35-1 Yagiyama-Kasumi, Taihaku, 983, Sendai, Japan
Search for more papers by this authorCorresponding Author
Robert R. Kay
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom
Jean-Paul Rieu, Laboratoire de Physique de la Matib̀eère Condensée et Nanostructures, Université de Lyon, Université de Lyon I, CNRS, UMR 5586, 43 Boulevard du 11 Nov. 1918, 69622 Villeurbanne Cedex, France
Robert R. Kay, MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom
Search for more papers by this authorAbstract
The collective motion of cells in a biological tissue originates from their individual responses to chemical and mechanical signals. The Dictyostelium slug moves as a collective of up to 100,000 cells with prestalk cells in the anterior 10–30% and prespore cells, intermingled with anterior-like cells (AL cells), in the posterior. We used traction force microscopy to measure the forces exerted by migrating slugs. Wild-type slugs exert frictional forces on their substratum in the direction of motion in their anterior, balanced by motive forces dispersed down their length. StlB− mutants lack the signal molecule DIF-1 and hence a subpopulation of AL cells. They produce little if any motive force in their rear and immediately break up. This argues that AL cells, but not prespore cells, are the motive cells in the posterior zone. Slugs also exert large outward radial forces, which we have analyzed during “looping” movement. Each time the anterior touches down after a loop, the outward forces rapidly develop, approximately normal to the almost stationary contact lines. We postulate that these forces result from the immediate binding of the sheath to the substratum and the subsequent application of outward “pressure,” which might be developed in several different ways. Cell Motil. Cytoskeleton 2009. © 2009 Wiley-Liss, Inc.
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