A Sensitive Surface-enhanced Raman Scattering Method for Determination of Melamine with Aptamer-modified Nanosilver Probe
Guiqing Wen
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorLianping Zhou
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorTingsheng Li
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorCorresponding Author
Aihui Liang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, ChinaSearch for more papers by this authorCorresponding Author
Zhiliang Jiang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, ChinaSearch for more papers by this authorGuiqing Wen
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorLianping Zhou
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorTingsheng Li
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Search for more papers by this authorCorresponding Author
Aihui Liang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, ChinaSearch for more papers by this authorCorresponding Author
Zhiliang Jiang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, China
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, Guangxi 541004, ChinaSearch for more papers by this authorAbstract
The small nanosilver was prepared by the sodium borohydride procedure. The aptamer was used to modify nanosilver to obtain a nanosilver-aptamer (AgssDNA) SERS probe for the determination of melamine. In pH 6.6 phosphate buffer solution and in the presence of NaCl, the AgssDNA probe specifically combined with melamine to release nanosilver particles that were aggregated to nanosilver clusters, which exhibited SERS effect at 240 cm−1. When melamine concentration increased, the nanosilver clusters increased, and the SERS intensity at 240 cm−1 increased. The increased SERS intensity ΔI240 cm−1 is linear to melamine concentration in the range of 6.3–403.6 μg·L−1, with a detection limit of 1.2 μg·L−1. This assay was applied to determination of melamine in milk, with satisfactory results.
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