Further Study on Separation of DNA Fragments by Capillary Electrophoresis by Quasi-interpenetrating Network of Polyacryamide and Polyvinylpyrrolidone with UV Detection
Qian Wang
Laboratory of Analytical Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
Search for more papers by this authorLi-Xin Dai
State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
Search for more papers by this authorQian Wang
Laboratory of Analytical Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
Search for more papers by this authorLi-Xin Dai
State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China
Search for more papers by this authorAbstract
Quasi-interpenetrating network of polyacrylamide (PAA) and polyvinylpyrrolidone (PVP) had been successfully used for single-base resolution of double-stranded DNA (0.76 for 123 bp/124 bp) and single-stranded DNA fragments (0.97 for 123 b/124 b) with UV detection. This quasi-IPN (interpenetrating network) sieving matrix showed low viscosity (23.5 mPa·s at 25 °C) and decreased with increasing temperature. This polymer also exhibited dynamically coating capacity and could be used in the uncoated capillary. The effects of temperature and electric field strength on the DNA separation of quasi-IPN matrix were also investigated and found that the temperature and electric field strength could markedly affected the mobility behavior of DNA fragments. This polymer matrix has also applied to separate the bigger DNA fragments by capillary electrophoresis with UV detection. Under the denaturing conditions, this matrix separated the samples with last fragment of 1353 base in 40 min, in which the doublet of 309/310 base was partial separated and the resolution was 0.88.
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