Ionic liquids as mobile phase additives for separation of nucleotides in high-performance liquid chromatography
Wen-Zhu Zhang
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
Search for more papers by this authorLi-Jun He
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
Search for more papers by this authorXia Liu
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
Search for more papers by this authorCorresponding Author
Sheng-Xiang Jiang
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
[email protected]; Tel.: 0931-8277652; Fax: 0931-8277088Search for more papers by this authorWen-Zhu Zhang
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
Search for more papers by this authorLi-Jun He
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
Search for more papers by this authorXia Liu
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
Search for more papers by this authorCorresponding Author
Sheng-Xiang Jiang
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China
[email protected]; Tel.: 0931-8277652; Fax: 0931-8277088Search for more papers by this authorAbstract
Ionic liquids are a type of salts that are liquid at low temperature (<100 °C). Because of their some special properties, they have been widely used as new “green solvents” for many chemical reactions and liquid-liquid extraction in the past several years. In this paper, a new method for the separation of nucleotides is developed and the essential feature of the method is that 1-alkyl-3-methylimidazolium salts are used as mobile phase additives, resulting in a baseline separation of nucleotides without need of gradient elution and need of organic solvent addition as currently used in RP-HPLC. This study shows the potential application of ionic liquids as mobile phase additives in reversed-phase liquid chromatography.
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