Prediction of Retention Time of Solutes under Linear Gradient Elution Conditions in RP-HPLC

A model for predicting retention tune of solutes under linear gradient elution conditions has been established. In this model, the theoretical expressions under different elution modes were derived and tested with the retention behaviors of p-hydroxybenzaldehyde, vanillin, biphenyl, phenanthrene in gradient elution. With taking into account the dwell time of the instrumental system, the theoretically predicted retention times agree well with those experimentally determined.