Mycobolome of Phialomyces Macrosporus Across OSMAC-Based Assorted Culture Media
Vitória Evelyn Teles de Jesus
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Contribution: Data curation (supporting), Investigation (equal), Methodology (equal), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorYasmin Alvarenga
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Contribution: Formal analysis (supporting), Investigation (supporting), Software (lead), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorElisangela F. Boffo
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Contribution: Formal analysis (supporting), Investigation (supporting), Software (lead), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorCorresponding Author
Regina Geris
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Search for more papers by this authorVitória Evelyn Teles de Jesus
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Contribution: Data curation (supporting), Investigation (equal), Methodology (equal), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorYasmin Alvarenga
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Contribution: Formal analysis (supporting), Investigation (supporting), Software (lead), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorElisangela F. Boffo
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Contribution: Formal analysis (supporting), Investigation (supporting), Software (lead), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorCorresponding Author
Regina Geris
Laboratório de Biotecnologia e Química de Microrganismos (LBQM), Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo S/n, Salvador, 40170-115 Brasil
Search for more papers by this authorAbstract
The fungus Phialomyces macrosporus was cultured using the One Strain Many Compounds (OSMAC) strategies to evaluate its metabolome. Variations in the nutrient culture media, culture regime, and cultivation parameters can significantly influence fungal extract quantity and chemical diversity. This study aimed to explore the mycobolome of P. macrosporus in five different culture media and two different cultivation conditions using NMR-based metabolomics. Principal component analysis (PCA) of 1H-NMR spectra revealed clear differentiation between these samples, highlighting the rice dextrose agar medium (RDA) and potato dextrose broth (PDB) as standard complex media for conducting a fungal metabolite screening program.
Graphical Abstract
Conflict of Interests
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available in the supplementary material of this article.
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References
- 1
- 1aS. Sanchez, A. L. Demain, Springer International Publishing, Cham 2017, 59–87;
- 1bR. Conrado, T. C. Gomes, G. S. C. Roque, A. O. De Souza, Antibiotics-Basel 2022, 11, 1604;
- 1cH. El-Gendi, A. K. Saleh, R. Badierah, E. M. Redwan, Y. A. El-Maradny, E. M. El-Fakharany, J. Fungi 2022, 8, 23.
- 2W. Y. Wang, T. T. Wang, Front. Microbiol. 2023, 141150023.
- 3A. A. Zhgun, Int. J. Mol. Sci. 2023, 24, 11184.
- 4O. Mosunova, J. C. Navarro-Muñoz, J. Collemare, Ó. Zaragoza, A. Casadevall, Elsevier, Oxford 2021, 458–476.
- 5B. Baral, A. Akhgari, M. Metsä-Ketelä, Synth. Syst. Biotechnol. 2018, 3, 163–178.
- 6B. C. Fan, D. Parrot, M. Blümel, A. Labes, D. Tasdemir, Mar. Drugs 2019, 17, 67.
- 7X. P. Zhang, X. W. Hou, D. Xu, M. Y. Xue, J. Y. Zhang, J. C. Wang, Y. L. Yang, D. W. Lai, L. G. Zhou, J. Fungi 2023, 9, 390.
- 8H. B. Bode, B. Bethe, R. Höfs, A. Zeeck, ChemBioChem 2002, 3, 619–627.
10.1002/1439-7633(20020703)3:7<619::AID-CBIC619>3.0.CO;2-9 CAS PubMed Web of Science® Google Scholar
- 9
- 9aL. Flores-Bocanegra, Z. Y. Al Subeh, J. M. Egan, T. El-Elimat, H. A. Raja, J. E. Burdette, C. J. Pearce, R. G. Linington, N. H. Oberlies, J. Nat. Prod. 2022, 85, 614–624;
- 9bZ. M. Huang, T. Bi, H. P. Jiang, H. W. Liu, Phytochem. Anal. 2023; 1-12. doi:10.1002/pca.3292;
- 9cA. Klitgaard, A. Iversen, M. R. Andersen, T. O. Larsen, J. C. Frisvad, K. F. Nielsen, Anal. Bioanal. Chem. 2014, 406, 1933–1943;
- 9dT. T. Lu, Y. Y. Liu, L. J. Zhou, Q. N. Liao, Y. Y. Nie, X. Y. Wang, X. L. Lei, P. Z. Hong, Y. Feng, X. Q. Hu, Y. Zhang, Front. Microbiol. 2023, 141144328;
- 9eA. E. Mohammed, H. Sonbol, S. S. Alwakeel, M. O. Alotaibi, S. Alotaibi, N. Alothman, R. S. Suliman, H. T. Ahmedah, R. Ali, Sci. Rep. 2021, 11, 4760;
- 9fK. F. Nielsen, T. O. Larsen, Front. Microbiol. 2015, 6, 71;
- 9gK. H. Shaker, M. M. Zohair, A. Z. Hassan, H. T. M. Sweelam, W. E. Ashour, Arch. Microbiol. 2022, 204, 661;
- 9hT. K. George, D. Devadasan, M. S. Jisha, Heliyon 2019, 5, e02484.
- 10M. D. Brandao, L. S. Abreu, R. Geris, Chem. Biodiversity 2019, 16, e1900353.
- 11M. Furui, T. Komatsubara, J. Kimura, N. Chiba, T. Mikawa, 1997.
- 12R. Geris, V. E. T. de Jesus, A. F. da Silva, M. Malta, Chem. Biodiversity 2024, 21, e202400556.
- 13R. Pan, X. L. Bai, J. W. Chen, H. W. Zhang, H. Wang, Front. Microbiol. 2019, 10, 294.
- 14H. N. Lyu, H. W. Liu, N. P. Keller, W. B. Yin, Nat. Prod. Rep. 2020, 37, 6–16.
- 15P. E. Hansen, Molecules 2021, 26, 2409.
- 16T. El-Elimat, M. Figueroa, H. A. Raja, T. N. Graf, S. M. Swanson, J. O. Falkinham, M. C. Wani, C. J. Pearce, N. H. Oberlies, Eur. J. Org. Chem. 2015, 2015, 109–121.
- 17R. Andersen, G. Buchi, B. Kobbe, A. L. Demain, J. Org. Chem. 1977, 42, 352–353.
- 18R. J. Cox, T. J. Simpson, Complex Enzymes in Microbial Natural Product Biosynthesis, Part B: Polyketides, Aminocoumarins and Carbohydrates, Academic Press, Amsterdam, Boston 2009, 49–78.
10.1016/S0076-6879(09)04603-5 Google Scholar
- 19K. M. J. de Mattos-Shipley, T. J. Simpson, Nat. Prod. Rep. 2023, 40, 174–201.
- 20B. FRANCK, The Biosynthesis of the Mycotoxins:A Study in Secondary Metabolism (Ed: P. S. Steyn), Academic Press 1980, 19580.
- 21A. F. Zhai, X. N. Zhu, X. L. Wang, R. Z. Chen, H. Wang, Eur. J. Pharmacol. 2013, 713, 58–67.
- 22D. Vesely, D. Vesela, R. Jelinek, Teratology 1992, 46, 131–136.
- 23S. K. Guru, A. S. Pathania, S. Kumar, D. Ramesh, M. Kumar, S. Rana, A. Kumar, F. Malik, P. R. Sharma, B. K. Chandan, S. Jaglan, J. P. Sharma, B. A. Shah, S. A. Tasduq, S. K. Lattoo, A. Faruk, A. K. Saxena, R. A. Vishwakarma, S. Bhushan, Cancer Res. 2015, 75, 2886–2896.
- 24K. M. VanderMolen, H. A. Raja, T. El-Elimat, N. H. Oberlies, Amb Express 2013, 3, 71.
