Seeds of Hyoscyamus muticus L. subsp. falezlez: Morpho-Anatomical Features, Phytochemical Investigation and Evidence for Antioxidant Activities
Sofia Ayari-Guentri
Département des Sciences de la Nature et de la Vie, Faculté des Sciences, Université d'Alger 1, Benyoucef Benkhedda, 02. Didouche Mourad, Algiers, Algeria
Laboratoire de Recherche sur les Zones Arides (LRZA), Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, Bab Ezzouar, Algiers, 16111 Algeria
Search for more papers by this authorSomia Saad
Centre de Recherche Scientifique et Technique sur les Régions Arides (CRSTRA), Biskra, Algeria
Search for more papers by this authorTassadit Ait Kettout
Laboratoire de Recherche sur les Zones Arides (LRZA), Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, Bab Ezzouar, Algiers, 16111 Algeria
Search for more papers by this authorRabéa Gaceb-Terrak
Laboratoire de Recherche sur les Zones Arides (LRZA), Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, Bab Ezzouar, Algiers, 16111 Algeria
Search for more papers by this authorCorresponding Author
Nadjette Djemouai
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaia, Ghardaïa, Algeria
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria
Search for more papers by this authorSofia Ayari-Guentri
Département des Sciences de la Nature et de la Vie, Faculté des Sciences, Université d'Alger 1, Benyoucef Benkhedda, 02. Didouche Mourad, Algiers, Algeria
Laboratoire de Recherche sur les Zones Arides (LRZA), Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, Bab Ezzouar, Algiers, 16111 Algeria
Search for more papers by this authorSomia Saad
Centre de Recherche Scientifique et Technique sur les Régions Arides (CRSTRA), Biskra, Algeria
Search for more papers by this authorTassadit Ait Kettout
Laboratoire de Recherche sur les Zones Arides (LRZA), Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, Bab Ezzouar, Algiers, 16111 Algeria
Search for more papers by this authorRabéa Gaceb-Terrak
Laboratoire de Recherche sur les Zones Arides (LRZA), Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, Bab Ezzouar, Algiers, 16111 Algeria
Search for more papers by this authorCorresponding Author
Nadjette Djemouai
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaia, Ghardaïa, Algeria
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Algiers, Algeria
Search for more papers by this authorAbstract
This work aims to describe the morpho-anatomy and determine the mineral composition of H. muticus L. subsp. falezlez seed, and investigate the influence of extraction solvents (hexane, petroleum ether, chloroform and methanol) on contents of total phenolic (TPC) and flavonoid (TFC) and the antioxidant activity (DPPH, ABTS, reducing power and phenanthroline). The mineral content analysis revealed the presence of different elements. The results indicated that the seed extract obtained by methanol exhibited the highest yield and TPC (82.07 % and 27.14±2.2 μg GAE/mg), while the chloroform extract had the maximum TFC (24.63±0.4 μg QE/mg). In addition, all seed extracts showed excellent performances on DPPH scavenging activity. However, methanolic extract was the most effective extraction solvent for scavenging ABTS free radicals (IC50<12.5 μg/mL). Chloroform and petroleum ether extracts were the most potent in phenanthroline activity. GC-MS analysis identified 28 phytochemical compounds in all extracts and 16 compounds were identified by HPLC/DAD analysis of the methanolic extract. The multivariate analysis results highlighted the influence of solvents on the phenolic composition and antioxidant activity of seed extracts. The results suggest that H. muticus L. seeds can be a promising source enriched with bioactive constituents for application in the pharmaceutical industry.
Graphical Abstract
Conflict of Interests
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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