Volume 21, Issue 10 e202400318
Research Article

Anthocyanin Profiles in the Tropical Fruits Eugenia jambolana and Inga edulis: A Comparative Study Using Paper Spray Ionization (PSI-MS), Tissue Spray Ionization (TSI-MS), and Direct Infusion (DI-MS)

Gesiane S. Lima

Corresponding Author

Gesiane S. Lima

Chemistry Institute, Federal University of Goias, 74690-900 Goiania (GO), Brazil

Contribution: Conceptualization (equal), Formal analysis (equal), ​Investigation (equal), Methodology (equal), Validation (equal), Writing - original draft (equal)

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Nerilson M. Lima

Nerilson M. Lima

Chemistry Institute, Federal University of Goias, 74690-900 Goiania (GO), Brazil

Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), ​Investigation (equal), Methodology (equal), Validation (equal), Writing - original draft (equal)

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Naará S. Balbino

Naará S. Balbino

Chemistry Institute, Federal University of Goias, 74690-900 Goiania (GO), Brazil

Contribution: ​Investigation (supporting), Methodology (supporting), Validation (supporting)

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Jean C. P. Sousa

Jean C. P. Sousa

Chemistry Institute, Federal University of Goias, 74690-900 Goiania (GO), Brazil

Contribution: ​Investigation (supporting), Methodology (supporting)

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Gabriel F. Santos

Gabriel F. Santos

Chemistry Institute, Federal University of Goias, 74690-900 Goiania (GO), Brazil

Contribution: Writing - review & editing (equal)

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Boniek G. Vaz

Boniek G. Vaz

Chemistry Institute, Federal University of Goias, 74690-900 Goiania (GO), Brazil

Contribution: Supervision (equal), Writing - review & editing (equal)

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First published: 29 July 2024
Citations: 2

Abstract

Paper spray ionization (PSI-MS) and tissue spray ionization (TSI-MS) mass spectrometry are simple and rapid ambient ionization mass spectrometry techniques that offer numerous advantages over conventional analysis methods. This study aims to analyze the efficiency of detecting anthocyanins from Eugenia jambolana fruit peel and Inga edulis seeds using PSI-MS, TSI-MS, and DI-MS (direct infusion). DI-MS exhibited high efficiency, detecting all compounds in abundance, with anthocyanins malvidin 3,5-O-diglucoside (1) and petunidin 3,5-O-diglucoside (2) being the most prevalent. PSI-MS, however, struggled to detect delphinidin 3-O-glucoside and showed lower abundances for compounds 1, 2, 3 (delphinidin 3,5-O-diglucoside), and 4 (petunidin 3-O-glucoside) compared to DI-MS, attributed to the technique's challenges with molecular weight and polarity. TSI-MS was least effective, detecting only compounds 1, 2, and 3 at low intensities. The overall unique compounds identified across techniques were 134, emphasizing the importance of comprehensively employing multiple methods to analyze anthocyanins in these edible plants.

Graphical Abstract

Conflict of Interests

The authors declare no conflict of interest.

Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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