Antitussive, Expectorant and Antipyretic Effect of the Ethanolic Extract of the Leaves of Momordica charantia L.
Tiago Feitosa Ribeiro
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorNathália Andrezza Carvalho de Souza
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorTarcísio Cícero de Lima Araújo
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorCícero André Ferreira Macedo
Program of Graduate Studies in Biotechnology, State University of Feira de Santana (UEFS), Bahia, Brazil
Search for more papers by this authorDr. Fabrício Souza Silva
Program of Graduate Studies in Biotechnology, State University of Feira de Santana (UEFS), Bahia, Brazil
Federal University of Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil
Search for more papers by this authorAndressa de Oliveira Siqueira
Federal University of Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil
Search for more papers by this authorDr. Pedro Modesto Nascimento Menezes
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Search for more papers by this authorDr. Camila Gomes de Melo
Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil
Search for more papers by this authorDr. Pedro José Rolim Neto
Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil
Search for more papers by this authorDr. Raimundo Gonçalves de Oliveira Junior
CiTCoM UMR 8038 CNRS, Faculté de Santé, Université Paris Cité, Paris, France
Search for more papers by this authorCorresponding Author
Dr. Henrique Douglas Melo Coutinho
Department of Biological Chemistry, Regional University of Cariri (URCA), Crato, Ceará, Brazil
Search for more papers by this authorDr. António Raposo
CBIOS (Research Center for Biosciences and Health Technologies), Universidade Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal
Search for more papers by this authorCorresponding Author
Dr. Larissa Araújo Rolim
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorTiago Feitosa Ribeiro
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorNathália Andrezza Carvalho de Souza
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorTarcísio Cícero de Lima Araújo
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorCícero André Ferreira Macedo
Program of Graduate Studies in Biotechnology, State University of Feira de Santana (UEFS), Bahia, Brazil
Search for more papers by this authorDr. Fabrício Souza Silva
Program of Graduate Studies in Biotechnology, State University of Feira de Santana (UEFS), Bahia, Brazil
Federal University of Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil
Search for more papers by this authorAndressa de Oliveira Siqueira
Federal University of Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil
Search for more papers by this authorDr. Pedro Modesto Nascimento Menezes
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Search for more papers by this authorDr. Camila Gomes de Melo
Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil
Search for more papers by this authorDr. Pedro José Rolim Neto
Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil
Search for more papers by this authorDr. Raimundo Gonçalves de Oliveira Junior
CiTCoM UMR 8038 CNRS, Faculté de Santé, Université Paris Cité, Paris, France
Search for more papers by this authorCorresponding Author
Dr. Henrique Douglas Melo Coutinho
Department of Biological Chemistry, Regional University of Cariri (URCA), Crato, Ceará, Brazil
Search for more papers by this authorDr. António Raposo
CBIOS (Research Center for Biosciences and Health Technologies), Universidade Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal
Search for more papers by this authorCorresponding Author
Dr. Larissa Araújo Rolim
Central for Analysis of Drugs, Medicines and Food (CAFMA), Federal University of Vale do São Francisco, Pernambuco, Brazil
Northeast Network of Biotechnology (RENORBIO), Federal Rural University of Pernambuco (UFRPE), Recife, Pernambuco, Brazil
Search for more papers by this authorAbstract
The traditional use of the M. charantia L. plant to treat coughs, fever and expectoration is widely practiced in different cultures, but its effectiveness and safety still require scientific investigation. This study sought to perform a chemical analysis and evaluate the antitussive, expectorant and antipyretic effects of the ethanolic extract of M. charantia leaves (EEMc) in rats and mice. The EEMc was subjected to chemical analysis by HPLC-DAD, revealing the presence of the flavonoids astragalin and isoquercetin. Acute oral toxicity in mice did not result in deaths, although changes in liver weight and stool consistency were observed. EEMc demonstrated an antitussive effect at doses of 100 and 300 mg/kg in mice subjected to cough induction by citric acid nebulization. Furthermore, it showed expectorant activity at a dose of 300 mg/kg, assessed based on the elimination of the phenol red marker in bronchoalveolar lavage. In the evaluation of antipyretic activity in rats, fever induced by Saccharomyces cerevisiae was reduced at all doses tested during the first hour after treatment. This innovative study identified the presence of astragalin and isoquercetin in EEMc and indicated that the extract has antitussive, expectorant and antipyretic properties. Therefore, EEMc presents itself as a promising option in herbal medicine for the treatment of respiratory symptoms and fever.
Graphical Abstract
Conflict of Interests
The authors have no conflict of interest to declare.
Open Research
Data Availability Statement
Data will be made available upon request.
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