Pulsed electromagnetic field stimulation of bone marrow cells derived from ovariectomized rats affects osteoclast formation and local factor production
Kyle Chang
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, Taiwan, Republic of China
Search for more papers by this authorCorresponding Author
Walter Hong-Shong Chang
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, Taiwan, Republic of China
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li 32023, Taiwan, Republic of China.Search for more papers by this authorYen-Hsin Yu
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, Taiwan, Republic of China
Search for more papers by this authorChung Shih
Institute of Biology and Anatomy, National Defense, Medical Center, Taipei, Taiwan, Republic of China
Search for more papers by this authorKyle Chang
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, Taiwan, Republic of China
Search for more papers by this authorCorresponding Author
Walter Hong-Shong Chang
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, Taiwan, Republic of China
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li 32023, Taiwan, Republic of China.Search for more papers by this authorYen-Hsin Yu
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, Taiwan, Republic of China
Search for more papers by this authorChung Shih
Institute of Biology and Anatomy, National Defense, Medical Center, Taipei, Taiwan, Republic of China
Search for more papers by this authorAbstract
This study examined the effects of a specific pulsed electromagnetic field (PEMF) stimulation on osteoclast formation in bone marrow cells from ovariectomized rats and to determine if the signal modulates the production of cytokines associated with osteoclast formation. Adult female Wistar rats were subjected to bilateral or sham ovariectomy, and primary bone marrow cells were harvested at 4 days (Subgroup I) and 7 days (Subgroup II) after surgery. Primary bone marrow cells were subsequently placed in chamber slides and set inside solenoids powered by a pulse generator (300 μs, 7.5 Hz) for 1 h per day for 9 days (OVX + PEMF group). Others (INT, SHAM, and OVX groups) were cultured under identical conditions, but no signal was applied. Recruitment and authentication of osteoclast-like cells were evaluated by determining multinuclear, tartrate-resistant acid phosphatase (TRAP) positive cells on day 10 of culture and by pit formation assay, respectively. The PEMF signal caused significant reductions in osteoclast formation in both Subgroups I (−55%) and II (−43%). Tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and interleukin 6 (IL-6) in OVX + PEMF group of Subgroup I were significantly reduced at 5, 7, and 9 days as compared to OVX group. The results found in this study suggest that osteoclastogenesis can be inhibited by PEMF stimulation, putatively due to a concomitant decrease in local factor production. Bioelectromagnetics 25:134–141, 2004. © 2004 Wiley-Liss, Inc.
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