Elevations of Neutrophil Proteinase 3 in Serum of Patients with Wegener's Granulomatosis and Polyarteritis Nodosa
Timothy J. Henshaw MD
University of Florida, Gainesville (current address: Goldsboro Rheumatology Associates, Goldsboro, NC)
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Search for more papers by this authorChristine C. Malone MS
University of Florida, Gainesville
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Search for more papers by this authorJoelle E. Gabay PhD
Cornell University Medical College
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Search for more papers by this authorCorresponding Author
Ralph C. Williams Jr. MD
Eminent Scholar and Chair, Marcia Whitney Schott Endowment, University of Florida, Gainesville.
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Department of Medicine, Division of Rheumatology, PO Box 100221, Gainesville, FL 32610Search for more papers by this authorTimothy J. Henshaw MD
University of Florida, Gainesville (current address: Goldsboro Rheumatology Associates, Goldsboro, NC)
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Search for more papers by this authorChristine C. Malone MS
University of Florida, Gainesville
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Search for more papers by this authorJoelle E. Gabay PhD
Cornell University Medical College
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Search for more papers by this authorCorresponding Author
Ralph C. Williams Jr. MD
Eminent Scholar and Chair, Marcia Whitney Schott Endowment, University of Florida, Gainesville.
Department of Medicine, Division of Rheumatology, University of Florida, Gainesville, and the Department of Pathology, Cornell University Medical College, New York, New York.
Department of Medicine, Division of Rheumatology, PO Box 100221, Gainesville, FL 32610Search for more papers by this authorAbstract
Objective. To determine serum levels of proteinase 3 (PR3) in normal subjects and patients with Wegener's granulomatosis (WG), polyarteritis nodosa (PAN), chronic renal failure, and systemic lupus erythematosus (SLE).
Methods. Serum levels of PR3 were measured by enzyme-linked immunosorbent assay employing a monospecific rabbit polyclonal antibody against PR3.
Results. Quantifiable levels of PR3 were detected in all serum samples examined, including those of normal subjects. Marked elevations of serum PR3, which decreased with treatment, were found in patients with active WG. Patients with PAN, SLE, and chronic renal failure also showed elevated levels of PR3.
Conclusion. Quantitating serum PR3 may be useful in the management of patients with WG and other connective tissue diseases.
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