Article
Full Access
Osteoarthritis-related changes in human articular cartilage
J. Vilamitjana-Amedee PhD,
M.-F. Harmand EsScD,
Corresponding Author
J. Vilamitjana-Amedee PhD
INSERM U306, Universite de Bordeaux II, Bor-deaux, France.
INSERM U306, Université de Bordeaux II, 146, rue Léo-Saignat, 33076 Bordeaux Cedex, FranceSearch for more papers by this authorM.-F. Harmand EsScD
INSERM U306, Universite de Bordeaux II, Bor-deaux, France.
Search for more papers by this authorJ. Vilamitjana-Amedee PhD,
M.-F. Harmand EsScD,
Corresponding Author
J. Vilamitjana-Amedee PhD
INSERM U306, Universite de Bordeaux II, Bor-deaux, France.
INSERM U306, Université de Bordeaux II, 146, rue Léo-Saignat, 33076 Bordeaux Cedex, FranceSearch for more papers by this authorM.-F. Harmand EsScD
INSERM U306, Universite de Bordeaux II, Bor-deaux, France.
Search for more papers by this authorAbstract
We report the use of immunologic methods for detecting specific alterations in human osteoarthritic cartilage. Monoclonal antibodies with specificities for proteoglycans and link proteins have been used to define immunogenic sites in these molecules. We have identified 1 site in the protein core, 1 site in the link proteins, and another site that is common to both proteoglycan and link proteins, which are not modified in osteoarthritic cartilage. In addition, an antigenic determinant in link proteins that is altered in osteoarthritic cartilage has been identified. These results suggest that the structure of the ternary complex (hyaluronic acid binding region-link proteins-hyaluronic acid) could be altered in osteoarthritic cartilage. These modifications may be due to a genetic defect or to partial enzymatic degradation of these sites.
References
- 1 Harmand MF, Vilamitjana J, Ducassou D: Osteoarthritis related changes in human articular cartilage: biochemical and immunological studies (abstract). Trans Orthop Res Soc 12: 305, 1987
- 2 Kohler G, Milstein C: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495–497, 1975
- 3 Caterson B, Baker JR, Christner JE, Kearne JF, Stohrer RC: Monoclonal antibodies directed against bovine nasal cartilage proteoglycan and link protein, Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. Edited by GJ Hammerling, U Hammerling, JF Kearney. Amsterdam, Elsevier/North Holland, 1981
- 4 Caterson B, Baker JR, Christner JE, Couchman JR: Immunological methods for the detection and determination of connective tissue proteoglycans. J Invest Dermatol 79: 45s–50s, 1982
- 5 Caterson B, Christner JE, Baker JR: Identification of a monoclonal antibody that specifically recognized corneal and skeletal keratan sulfate: monoclonal antibodies to cartilage proteoglycan. J Biol Chem 258: 8848–8854, 1983
- 6 Caterson B, Christner JE, Baker JR, Couchman JR: Production and characterization of monoclonal antibodies directed against connective tissue proteoglycans. Fed Proc 44: 386–390, 1985
- 7 Caterson B, Baker JR, Christner JE, Lee Y, Lentz M: Monoclonal antibodies as probes for determining the microheterogeneity of the link proteins of cartilage proteoglycan. J Biol Chem 260: 1348–1356, 1985
- 8 Couchman JR, Caterson B, Christner JE, Baker JR: Mapping by monoclonal antibody detection of glycosaminoglycans in connective tissues. Nature 307: 650–652, 1984
- 9 Dorfman A, Hall T, Ho PL, Fitch F: Clonal antibodies to chick cartilage chondroitin sulfate proteoglycan. Proc Natl Acad Sci USA 76: 4847–4851, 1980
- 10 Jenkins RB, Hall T, Dorfman A: Chondroitin 6 sulfate oligosaccharides as immunological determinants of chick proteoglycans. J Biol Chem 256: 8279–8282, 1981
- 11 Glant TT, Mikecz K, Poole AR: Monoclonal antibodies to different protein-related epitopes of human articular cartilage proteoglycans. Biochem J 234: 31–41, 1986
- 12 Kresina TF, Malemud CJ, Moskowitz RW: Analysis of osteoarthritic cartilage using monoclonal antibodies reactive with rabbit proteoglycan. Arthritis Rheum 29: 863–871, 1986
- 13 Thonar EJ-MA, Lenz ME, Klintworth GK, Caterson B, Pachman LM, Glickman P, Katz R, Huff J, Kuettner KE: Quantification of keratan sulfate in blood as a marker of cartilage catabolism. Arthritis Rheum 28: 1367–1376, 1985
- 14 Bitter T, Muir HM: A modified uronic acid carbazole reaction. Anal Biochem 4: 330–334, 1962
- 15 Lowry OH, Rosebrough NJ, Farr AL, Randall RT: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951
- 16 Fazekas de Saint Groth S, Scheidegger D: Production of monoclonal antibodies: strategy and tactics. J Immunol Methods 35: 1–21, 1980
- 17 Saunders GC: Immunoassays in the Clinical Laboratory. Edited by RM Nakanura, WR Dito, ES Tucker. New York, Alan R. Liss, 1979
- 18 Laurent AG, Gruest J, Krust B, Montagnier L: Purification of monoclonal antibodies on DEAE trisacryl M. Hybridoma 1: 313–322, 1982
- 19 Salacinski PRP, McLean C, Sykes JEC, Clement-Jones V-V, Lowry PJ: Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3α,6α diphenyl glycoluril (IODOGEN). Anal Biochem 117: 136–146, 1981
- 20 Keiser HD: A solid phase immunoassay for the binding of cartilage proteoglycan to hyaluronic acid. Anal Biochem 160: 462–467, 1987
- 21 Huet J, Sentenac A, Fromageot P: Spot immunodetection of conserved determinants in eukaryotic RNA polymerases. J Biol Chem 257: 2613–2618, 1982
- 22 Heinegard D, Sommarin Y, Hedbom E, Wieslander J, Larsson B: Assay of proteoglycan populations using agarose-polyacrylamide gel electrophoresis. Anal Biochem 151: 41–48, 1985
- 23 Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970
- 24 Vilamitjana J, Barreau C: A monoclonal antibody directed against a small subunit of RNA polymerase B blocks the initiation step. Eur J Biochem 162: 317–323, 1987
- 25 Hawkes R, Niday E, Gordon J: A dot immunobinding assay for monoclonal and other antibodies. Anal Biochem 119: 142–147, 1982
- 26 Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 252: 1102–1106, 1977
- 27 Hardingham TE, Ewins RJF, Muir H: Cartilage proteoglycans: structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronate. Biochem J 157: 127–143, 1976
- 28 Calabro T, Caterson B, Hascall VC: Evidence for common epitopes in both proteoglycan core substructures and link protein (abstract). Trans Orthop Res Soc 12: 62, 1988
- 29 Neame PJ, Perin JP, Bonnet F, Christner JE, Jolles P, Baker JR: An amino acid sequence common to both cartilage proteoglycan and link protein. J Biol Chem 260: 12402–12404, 1986
- 30 Campbell IK, Roughley PJ, Mort JS: The action of human articular cartilage metalloproteinase on proteoglycan and link protein. Biochem J 237: 117–122, 1986
