Characterization of latent and active forms of cartilage proteinases produced by normal immature rabbit articular cartilage in tissue culture
Elizabeth C. Cartwright PhD
Research Fellow
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Elizabeth C. Cartwright was a Queen Elizabeth II Research Fellow.
Search for more papers by this authorIan K. Campbell BSc (Hons)
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Search for more papers by this authorMargaret L. Britz PhD
Department of Microbiology, University of Melbourne, Parkville, Victoria, Melbourne, Australia
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Search for more papers by this authorJohn D. Sandy PhD
Terence and Mathilda Kennedy Institute of Rheumatology, 6 Bute Gardens, Hammersmith, London, UK
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Search for more papers by this authorCorresponding Author
Dennis A. Lowther PhD
Professor of Biochemistry
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Monash University, Clayton, Victoria, 3168, AustraliaSearch for more papers by this authorElizabeth C. Cartwright PhD
Research Fellow
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Elizabeth C. Cartwright was a Queen Elizabeth II Research Fellow.
Search for more papers by this authorIan K. Campbell BSc (Hons)
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Search for more papers by this authorMargaret L. Britz PhD
Department of Microbiology, University of Melbourne, Parkville, Victoria, Melbourne, Australia
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Search for more papers by this authorJohn D. Sandy PhD
Terence and Mathilda Kennedy Institute of Rheumatology, 6 Bute Gardens, Hammersmith, London, UK
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Search for more papers by this authorCorresponding Author
Dennis A. Lowther PhD
Professor of Biochemistry
Department of Biochemistry, Monash University, Clayton, 3168, Victoria, Australia.
Monash University, Clayton, Victoria, 3168, AustraliaSearch for more papers by this authorAbstract
Cultured tissue slices from normal immature rabbit articular cartilage released latent neutral metal-loproteinases into serum-free medium. On activation with 4-aminophenylmercuric acetate, these metalloproteinases could degrade collagen, proteoglycan, and gelatin. Also produced were an acid proteinase with the properties of cathepsin D and an inhibitor of the neutral metalloproteinases. The appearance of both the proteinases and the inhibitor in the culture medium could be prevented by incubation of cultures with cycloheximide. The active and latent forms of the proteinases were characterized using Ultrogel AcA 54 chromatography.
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