Thermoplastic Polyurethane/Polylactic Acid Blend Foams Loaded With Curcuma longa L. and Hypericum perforatum Extracts Towards Wound Dressing Applications
Corresponding Author
Aleksandra Bužarovska
Ss Cyril and Methodius University in Skopje, Faculty of Technology and Metallurgy, Skopje, North Macedonia
Correspondence:
Aleksandra Bužarovska ([email protected])
Sorina Dinescu ([email protected])
Contribution: Investigation (lead), Methodology (lead), Resources (lead), Writing - original draft (lead)
Search for more papers by this authorJasmina Petreska Stanoeva
Ss. Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Institute of Chemistry, Skopje, North Macedonia
Contribution: Investigation (equal)
Search for more papers by this authorPredrag Karamanolevski
Kemet Electronics Macedonia, Technological Industrial Development Zone No. Skopje 1/NN Ilinden—Skopje, Skopje, North Macedonia
Contribution: Investigation (supporting)
Search for more papers by this authorAndreea Daniela Popa
Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania
Contribution: Investigation (equal), Methodology (equal)
Search for more papers by this authorCorresponding Author
Sorina Dinescu
Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania
The Research Institute of the University of Bucharest (ICUB), Bucharest, Romania
Correspondence:
Aleksandra Bužarovska ([email protected])
Sorina Dinescu ([email protected])
Contribution: Investigation (equal), Methodology (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorLuc Avérous
BioTeam/ICPEES-ECPM, UMR CNRS 7515, Université de Strasbourg, Strasbourg Cedex, France
Contribution: Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Aleksandra Bužarovska
Ss Cyril and Methodius University in Skopje, Faculty of Technology and Metallurgy, Skopje, North Macedonia
Correspondence:
Aleksandra Bužarovska ([email protected])
Sorina Dinescu ([email protected])
Contribution: Investigation (lead), Methodology (lead), Resources (lead), Writing - original draft (lead)
Search for more papers by this authorJasmina Petreska Stanoeva
Ss. Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Institute of Chemistry, Skopje, North Macedonia
Contribution: Investigation (equal)
Search for more papers by this authorPredrag Karamanolevski
Kemet Electronics Macedonia, Technological Industrial Development Zone No. Skopje 1/NN Ilinden—Skopje, Skopje, North Macedonia
Contribution: Investigation (supporting)
Search for more papers by this authorAndreea Daniela Popa
Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania
Contribution: Investigation (equal), Methodology (equal)
Search for more papers by this authorCorresponding Author
Sorina Dinescu
Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania
The Research Institute of the University of Bucharest (ICUB), Bucharest, Romania
Correspondence:
Aleksandra Bužarovska ([email protected])
Sorina Dinescu ([email protected])
Contribution: Investigation (equal), Methodology (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorLuc Avérous
BioTeam/ICPEES-ECPM, UMR CNRS 7515, Université de Strasbourg, Strasbourg Cedex, France
Contribution: Writing - review & editing (equal)
Search for more papers by this authorABSTRACT
This study aimed to fabricate and evaluate TPU/polylactic acid foams loaded with natural extracts of Curcuma longa L. (C) and Hypericum perforatum (H) for WD application. In the frame of the structure/properties relationships study, different and complementary analyzes for WD materials, such as water contact angles measurements, water vapor transmission rate, water absorption, and extracts release studies were performed, in connection with the structural and thermal characterizations performed using scanning electron microscopy, differential scanning calorimetry and Fourier transform spectroscopy. The water absorption capacity of the blank blend foam and foams loaded with extracts (C and H) was found to be in the range of 8.31% ± 0.27, 90.7% ± 4.1 and 164% ± 4.0%, respectively. The loaded foams presented cumulative extracts release of 45.5% ± 2% (C) and 100% ± 1.1% (H) within 96 h and acceptable water vapor transmission rate in the range of 2088 ± 66 and 3520 ± 991 g·m−2·24 h−1, respectively. Summary, the investigated foams, enriched with both extracts, showed excellent performances in agreement with WD materials requirements, higher cell viability and proliferation compared to the reference foams, as well as non-cytotoxic effects on human fibroblasts in experimental conditions.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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Data S1. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1 M. Minsart, S. Van Vlierberghe, P. Dubruel, and A. Mignon, “Commercial Wound Dressings for the Treatment of Exuding Wounds: An In-Depth Physico-Chemical Comparative Study,” Burns & Trauma 10, no. 2022 (2022): tkac024.
- 2 H. M. Nguyen, T. T. N. Le, A. T. Nguyen, H. N. T. Le, and T. T. Pham, “Biomedical Materials for Wound Dressing: Recent Advances and Applications,” Royal Society of Chemistry Advances 13 (2023): 5509–5528.
- 3 S. Hema, U. Vaani, D. Gayathri, B. Niranjan, C. Smitha, and S. Sreedha, “Bio-Based Polymers Containing Traditional Medicinal Fillers for Wound Healing Applications-An Evaluation of Neoteric Development and Future Perspectives,” Biotechnology Journal 18 (2023): e2300006.
- 4 S. Wendels and L. Avérous, “Biobased Polyurethanes for Biomedical Applications,” Bioactive Materials 6 (2021): 1083–1106.
- 5 R. Kaur, P. Singh, S. Tanwar, G. Varshney, and S. Yadav, “Assessment of Bio-Based Polyurethanes: Perspective on Applications and Bio-Degradation,” Macromolecules 2 (2022): 284–314.
- 6 M. Li, J. Chen, M. Shi, H. Zhang, P. X. Ma, and B. Guo, “Electroactive Anti-Oxidant Polyurethane Elastomers With Shape Memory Property as Non-Adherent Wound Dressing to Enhance Wound Healing,” Chemical Engineering Journal 375 (2019): 121999.
- 7 J. G. Lundin, G. C. Daniels, C. L. McGann, et al., “Multi-Functional Polyurethane Hydrogel Foams With Tunable Mechanical Properties for Wound Dressing Applications,” Macromolecular Materials and Engineering 302 (2017): 1600375.
- 8 Y. Ding, Z. Sun, R. Shi, et al., “Integrated Endotoxin Adsorption and Antibacterial Properties of Cationic Polyurethane Foams for Wound Healing,” Mater. Interfaces 11 (2019): 2860–2869.
- 9 C. Iga, T. Agata, L. Marcin, F. Natalia, and K. L. Justyna, “Ciprofloxacin-Modified Degradable Hybrid Polyurethane-Polylactide Porous Scaffolds Developed for Potential Use as an Antibacterial Scaffold for Regeneration of Skin,” Polymers 12 (2020): 171.
- 10 V. De Stefano, S. Khan, and A. Tabada, “Applications of PLA in Modern Medicine,” Engineered Regeneration 1 (2020): 76.
- 11 S. Milovanovic, J. Pajnik, and I. Lukic, “Tailoring of Advanced Poly(Lactic Acid)-Based Materials: A Review,” Journal of Applied Polymer Science 139 (2022): 51839.
- 12 A. Bužarovska, A. Selaru, M. Serban, et al., “Biobased Multiphase Foams With ZnO for Wound Dressing Applications,” Journal of Materials Science 58 (2023): 17594–17609.
- 13 S. Li, Y. Zhang, X. Ma, et al., “Antimicrobial Lignin-Based Polyurethane/Ag Composite Foams for Improving Wound Healing,” Biomacromolecules 23 (2022): 1622–1632.
- 14 A. Bužarovska, S. Dinescu, A. D. Lazar, et al., “Nanocomposite Foams Based on Flexible Biobased Thermoplastic Polyurethane and ZnO Nanoparticles as Potential Wound Dressing Materials,” Materials Science & Engineering. C, Materials for Biological Applications 104 (2019): 109893.
- 15 J. A. Ataide, B. Zanchetta, É. M. Santos, et al., “Nanotechnology-Based Dressings for Wound Management,” Pharmaceuticals 15 (2022): 1286.
- 16 M. Firoozbahr, P. Kingshott, E. A. Palombo, and B. Zaferanloo, “Recent Advances in Using Natural Antibacterial Additives in Bioactive Wound Dressings,” Pharmaceutics 15 (2023): 644.
- 17 L. M. C. Ferreira, E. S. Bandeira, M. F. Gomes, et al., “Polyacrylamide Hydrogel Containing Calendula Extract as a Wound Healing Bandage: In Vivo Test,” International Journal of Molecular Sciences 24 (2023): 3806.
- 18 E. Tamahkar, B. Özkahraman, Z. Özbaş, et al., “ Aloe vera-Based Antibacterial Porous Sponges for Wound Dressing Applications,” Journal of Porous Materials 28 (2021): 741–750.
- 19 A. Gavan, L. Colobatiu, D. Hanganu, et al., “Development and Evaluation of Hydrogel Wound Dressings Loaded With Herbal Extracts,” PRO 10 (2022): 242.
- 20 F. Tajik, N. Eslahi, A. Rashidi, and M. M. Rad, “Hybrid Antibacterial Hydrogels Based on PVP and Keratin Incorporated With Lavender Extract,” Journal of Polymer Research 28 (2021): 316.
- 21 A. B. García-Hernández, E. Morales-Sánchez, B. M. Berdeja-Martínez, et al., “PVA-Based Electrospun Biomembranes With Hydrolyzed Collagen and Ethanolic Extract of Hypericum perforatum for Potential Use as Wound Dressing: Fabrication and Characterization,” Polymers 2022 (1981): 12.
- 22 G. Isopencu, I. Deleanu, C. Busuioc, et al., “Bacterial Cellulose—Carboxymethylcellulose Composite Loaded With Turmeric Extract for Antimicrobial Wound Dressing Applications,” International Journal of Molecular Sciences 24 (2023): 1719.
- 23 J. G. Merrell, S. W. McLaughlin, L. Tie, C. T. Laurencin, A. F. Chen, and L. S. Nair, “Curcumin-Loaded Poly(Ε-Caprolactone) Nanofibres: Diabetic Wound Dressing With Anti-Oxidant and Anti-Inflammatory Properties,” Clinical and Experimental Pharmacology & Physiology 36 (2009): 1149–1156.
- 24 M. M. Rahaman, A. Rakib, S. Mitra, et al., “The Genius Curcuma and Inflammation: Overview of the Pharmacological Perspectives,” Plants 10 (2021): 63.
- 25 U. Wölfle, G. Seelinger, and C. M. Schempp, “Topical Application of St.John's Wort (Hypericum perforatum),” Planta Medica 80 (2014): 109–120.
- 26 A. Carrubba, S. Lazzara, A. Giovino, G. Ruberto, and E. Napoli, “Content Variability of Bioactive Secondary Metabolites in Hypericum perforatum L,” Phytochemistry Letters 46 (2021): 71–78.
- 27 B. Kocaadam and N. Şanlier, “Curcumin, an Active Component of Turmeric (Curcuma longa), and its Effects on Health,” Critical Reviews in Food Science and Nutrition 57 (2017): 2889–2895.
- 28 J. Zhang, L. Gao, J. Hu, et al., “Hypericin: Source, Determination, Separation, and Properties,” Separation and Purification Reviews 51 (2020): 1–10.
- 29 J. Niesiobędzka and J. Datta, “Challenges and Recent Advances in Bio-Based Isocyanate Production,” Green Chemistry 25 (2023): 2482–2504.
- 30 S. Lemouzy, A. Delavarde, F. Lamaty, X. Bantreil, J. Pinaud, and S. Caillol, “Lignin-Based Bisguaiacol Diisocyanate: A Green Route for the Synthesis of Biobased Polyurethanes,” Green Chemistry 25 (2023): 4833–4839.
- 31 E. W. Fisher, H. J. Sterzel, and G. Wagner, “Investigation of the Structure of Solution Grown Crystals of Lactide Copolymers by Means of Chemical Reactions,” Colloid and Polymer Science 251 (1973): 980.
- 32 ASTM standard E96, “Standard Test Methods for Water Vapour Transmission Materials. ASTM International,” (2000), http://www.astm.org.
- 33 A. Geethanjali, P. Lalitha, and M. J. Firdhouse, “Analysis of Curcumin Content of Turmeric Samples from Various States of India,” International Journal of Pharma and Chemical Research 2 (2016): 55.
- 34 D. Gitea, M. Şipos, T. Mircea, and B. Pasca, “The Analysis of Alcoholic Extracts of Hypericum Species by UV/VIS Spectrophotometry,” Analele Universităţii Din Oradea—Fascicula Biologie 17 (2010): 111.
- 35 A. Sharma, A. Mittal, V. Puri, P. Kumar, and I. Singh, “Curcumin-Loaded, Alginate–Gelatin Composite Fibers for Wound Healing Applications,” 3 Biotech 10 (2020): 464.
- 36 T. M. Sampath Udeni Gunathilake, Y. C. Ching, C. H. Chuah, et al., “Influence of a Nonionic Surfactant on Curcumin Delivery of Nanocellulose Reinforced Chitosan Hydrogel,” International Journal of Biological Macromolecules 118 (2018): 1055–1064.
- 37 K. Taraj, L. Ciko, I. Malollari, et al., “Eco-Extraction of Essential Oil from Albanian Hypericum perforatum L. and Characterisation by Spectroscopy Techniques,” Journal of Environmental Protection and Ecology 20 (2019): 188.
- 38 G. S. Nikolic and S. Z. Zlatkovic, “Assaying the Variation in Secondary Metabolites of St. John's Wort for Its Better Use as an Antibiotic,” Journal of Medicinal Plant Research 4 (2010): 211.
- 39 L. M. Gradinaru, M. Barbalata-Mandru, A. A. Enache, C. M. Rimbu, G. I. Badea, and M. Aflor, “Chitosan Membranes Containing Plant Extracts: Preparation, Characterization and Antimicrobial Properties,” International Journal of Molecular Sciences 24 (2023): 8673.
- 40 I. M. Pelin, M. Silion, I. Popescu, C. M. Rîmbu, G. Fundueanu, and M. Constantin, “Pullulan/Poly(vinyl alcohol) Hydrogels Loaded With Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications,” Pharmaceutics 15 (2023): 1674.
- 41 M. R. Sato, J. A. Oshiro-Junior, C. F. Rodero, et al., “Enhancing Antifungal Treatment of Candida Albicans With Hypericin-Loaded Nanostructured Lipid Carriers in Hydrogels: Characterization, In Vitro, and In Vivo Photodynamic Evaluation,” Pharmaceuticals 16 (2023): 1094.
- 42 E. Mitani, Y. Ozaki, and H. Sato, “Two Types of C O⋯HO Hydrogen Bonds and OH⋯OH (Dimer, Trimer, Oligomer) Hydrogen Bonds in PVA With 88% Saponification/PMMA and PVA With 99% Saponification/PMMA Blends and Their Thermal Behavior Studied by Infrared Spectroscopy,” Polymer 246 (2022): 124725.
- 43 R. Seymour, G. Estes, and S. L. Cooper, “Infrared Studies of Segmented Polyurethan Elastomers. I. Hydrogen Bonding,” Macromolecules 3 (1970): 579–583.
- 44 C. L. Zhang, J. Hu, and S. J. Chen, “Theoretical Study of Hydrogen Bonding Interactions on MDI-Based Polyurethane,” Journal of Molecular Modeling 16 (2010): 1391–1399.
- 45 M. Raja, S. H. Ryu, and A. M. Shanmugharaj, “Thermal, Mechanical and Electroactive Shape Memory Properties of Polyurethane (PU)/Poly (Lactic Acid) (PLA)/CNT Nanocomposites,” European Polymer Journal 49 (2013): 3492–3500.
- 46 A. R. Unnithan, G. Gnanasekaran, Y. Sathishkumar, Y. S. Lee, and C. S. Kim, “Electrospun Antibacterial Polyurethane–Cellulose Acetate–Zein Composite Mats for Wound Dressing,” Carbohydrate Polymers 102 (2014): 884–892.
- 47 G. Siboni, H. Weitman, D. Freeman, Y. Mazur, Z. Malik, and B. Ehrenberg, “The Correlation Between Hydrophilicity of Hypericins and Helianthrone: Internalization Mechanisms, Subcellular Distribution and Photodynamic Action in Colon Carcinoma Cells,” Photochemical & Photobiological Sciences 1 (2002): 483–491.
- 48 D. G. Pyun, H. S. Yoon, H. Y. Chung, et al., “Evaluation of AgHAP-Containing Polyurethane Foam Dressing for Wound Healing: Synthesis, Characterization, In Vitro and In Vivo Studies,” Journal of Materials Chemistry B 3 (2015): 7752–7763.
- 49 X. Liu, Y. Niu, K. C. Chen, and S. Chen, “Mater,” Materials Science and Engineering: C 71 (2017): 289–297.
- 50 P. Mistry, R. Chhabra, S. Muke, et al., “Fabrication and Characterization of Starch-TPU Based Nanofibers for Wound Healing Applications,” Materials Science and Engineering: C 119 (2021): 111316.
- 51 W. Liang, N. Ni, Y. Huang, and C. Lin, “An Advanced Review: Polyurethane-Related Dressings for Skin Wound Repair,” Polymers 15 (2023): 4301.
- 52
P. Zahedi, I. Rezaeian, S. O. Ranaei-Siadat, S. H. Jafari, and P. Supaphol, “A Review on Wound Dressings With an Emphasis on Electrospun Nanofibrous Polymeric Bandages,” Polymers for Advanced Technologies 1 (2010): 77–95.
10.1002/pat.1625 Google Scholar
- 53 G. Rezvan, G. Pircheraghi, and R. J. Bagheri, “Curcumin Incorporated PVA-Borax Dual Delivery Hydrogels as Potential Wound Dressing Materials—Correlation Between Viscoelastic Properties and Curcumin Release Rate,” Applied Polymer Science 135 (2018): 46734.
- 54 A. Abdul Samat, Z. A. Abdul Hamid, M. Jaafar, C. C. Ong, and B. H. Yahaya, “Investigation of the In Vitro and In Vivo Biocompatibility of a Three-Dimensional Printed Thermoplastic Polyurethane/Polylactic Acid Blend for the Development of Tracheal Scaffolds,” Bioengineering 10 (2023): 394.
- 55 A. Lis-Bartos, A. Smieszek, K. Frańczyk, and K. Marycz, “Fabrication, Characterization, and Cytotoxicity of Thermoplastic Polyurethane/Poly(Lactic Acid) Material Using Human Adipose Derived Mesenchymal Stromal Stem Cells (hASCs),” Polymers 10 (2018): 1073.
- 56 L. Sleiman, A. D. Lazăr, M. Albu-Kaya, et al., “Development and Investigation of an Innovative 3D Biohybrid Based on Collagen and Silk Sericin Enriched With Flavonoids for Potential Wound Healing Applications,” Polymers 16 (2024): 1627.
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