In this work, a molecularly imprinted sensor based on a composite of Ti₃C₂T_x (MXene) and carboxyl functionalized multiwall carbon nanotubes (COOH-MWCNT) is prepared for bilirubin (BR) detection in human serum. The surface morphology and structure of the synthesized composite are characterized via field emission scanning electron microscopy (FESEM) along with elemental dispersive X-ray analysis (EDX) and X-ray diffraction (XRD). The formation of the BR imprint is confirmed via FESEM, atomic force microscopy (AFM), and electrochemical methods such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectrometry (EIS) under optimized experimental parameters. The BR imprinted electrode is prepared on the MXene/COOH-MWCNT composite-modified electrode by using CV in 6 mM ortho-phenylenediamine (o-PD) containing 2 mM BR in sodium acetate buffer solution (pH 5, 0.1 M). The linear range, limit of detection (LOD) and sensitivity are calculated as 0.1–20 mg/dL, 0.002 mg/dL, and 11.7 μA μM⁻¹ cm⁻², respectively. In addition, selectivity, reusability, reproducibility, and stability studies are performed on the prepared electrode.