Synthesis of cross-linked diazaborine-based polymeric microparticles with antiquorum sensing, anti-swarming, antimicrobial, and antibiofilm properties
Corresponding Author
Alfred Ngenge Tamfu
Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, Ngaoundere, Cameroon
Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, Mugla, Turkey
Correspondence
Alfred Ngenge Tamfu, Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, 454, Ngaoundere, Cameroon.
Email: [email protected]
Hüseyin Çiçek, Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, 48000, Mugla, Turkey.
Email: [email protected]
Contribution: Conceptualization (equal), Investigation (equal), Methodology (equal), Writing - original draft (lead), Writing - review & editing (equal)
Search for more papers by this authorGökhan Kocak
Department of Chemistry and Chemical Process Technologies, Vocational School of Higher Education, Adiyaman University, Adiyaman, Turkey
Contribution: Conceptualization (equal), Investigation (equal), Methodology (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorOzgur Ceylan
Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, Mugla, Turkey
Contribution: Formal analysis (equal), Methodology (equal), Writing - review & editing (equal)
Search for more papers by this authorFunda Valentine
Department of Chemistry, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey
Contribution: Conceptualization (equal), Investigation (equal), Methodology (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorVural Bütün
Department of Chemistry, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey
Contribution: Funding acquisition (equal), Resources (equal), Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Hüseyin Çiçek
Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, Mugla, Turkey
Correspondence
Alfred Ngenge Tamfu, Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, 454, Ngaoundere, Cameroon.
Email: [email protected]
Hüseyin Çiçek, Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, 48000, Mugla, Turkey.
Email: [email protected]
Contribution: Funding acquisition (equal), Resources (equal), Supervision (lead), Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Alfred Ngenge Tamfu
Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, Ngaoundere, Cameroon
Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, Mugla, Turkey
Correspondence
Alfred Ngenge Tamfu, Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, 454, Ngaoundere, Cameroon.
Email: [email protected]
Hüseyin Çiçek, Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, 48000, Mugla, Turkey.
Email: [email protected]
Contribution: Conceptualization (equal), Investigation (equal), Methodology (equal), Writing - original draft (lead), Writing - review & editing (equal)
Search for more papers by this authorGökhan Kocak
Department of Chemistry and Chemical Process Technologies, Vocational School of Higher Education, Adiyaman University, Adiyaman, Turkey
Contribution: Conceptualization (equal), Investigation (equal), Methodology (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorOzgur Ceylan
Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School, Mugla Sitki Kocman University, Mugla, Turkey
Contribution: Formal analysis (equal), Methodology (equal), Writing - review & editing (equal)
Search for more papers by this authorFunda Valentine
Department of Chemistry, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey
Contribution: Conceptualization (equal), Investigation (equal), Methodology (equal), Writing - original draft (equal), Writing - review & editing (equal)
Search for more papers by this authorVural Bütün
Department of Chemistry, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey
Contribution: Funding acquisition (equal), Resources (equal), Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Hüseyin Çiçek
Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, Mugla, Turkey
Correspondence
Alfred Ngenge Tamfu, Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, 454, Ngaoundere, Cameroon.
Email: [email protected]
Hüseyin Çiçek, Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, 48000, Mugla, Turkey.
Email: [email protected]
Contribution: Funding acquisition (equal), Resources (equal), Supervision (lead), Writing - review & editing (equal)
Search for more papers by this authorFunding information: Scientific and Technological Research Council of Turkey, Grant/Award Number: 213M181
Abstract
Transmission of infectious diseases and resistance by microorganisms is a global health threat. Antimicrobial polymers are increasingly being developed as biocides with greater applications than traditional antibiotics. In this study, 3-(potassiumsulfonyl)propyl methacrylate (SPMA) monomer, ethylene glycol dimethacrylate cross-linker and ammonium persulfate initiator were reacted to yield poly[3-(potassiumsulfonyl)propyl methacrylate] microgels (PSPMA). Surface modification of PSPMA with thionyl chloride yielded poly[3-(chlorosulfonyl)propyl methacrylate microparticle] (PSPMA-Cl) which was reacted with hydrazinehydrate to obtain poly[3-(hydrazinylsulfonyl)propyl methacrylate] microparticles (PSPMA-NH2). Finally, 2-formyl benzeneboronic acid was added to give the final product poly[3-((1-hydroxybenzo(d)(1,2,3)diazaborinin-2(1H)-yl)sulfonyl)propyl methacrylate] (PSPMA-DAZ) microparticles. The modified microparticles were characterized using attenuated total reflectance-Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and energy-dispersive x-ray analyses. The polymeric microparticles showed good antibiofilm activity against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Candida albicans ATCC 10239 and Candida tropicalis ATCC 13803 while low to moderate biofilm inhibitions were observed against Listeria monocytogenes ATCC 7644, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853 and Salmonella typhi ATCC 14028. Surface group modification from PSPMA, PSPMA-Cl, PSPMA-NH2 to PSPMA-DAZ increased violacein inhibition, quorum-sensing inhibition and antimicrobial activity but reduced swarming. These polymer microparticles can be used to reduce spread of microbial infections and resistance.
CONFLICT OF INTEREST
The authors have no relevant financial or non-financial interests to disclose.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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