Microwave-assisted synthesis of chitosan biguanidine hydrochloride and its antioxidant activity in vitro
Qianwen Liu
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorYuan Li
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorXin Jin
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorQin Peng
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorCorresponding Author
Xiaofei Liu
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Correspondence to: X. F. Liu (E-mail: [email protected])Search for more papers by this authorYuntang Wu
Department of Health Statistics, College of Public Health, Tianjin Medical University, Tianjin, 300070 People's Republic of China
Search for more papers by this authorQianwen Liu
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorYuan Li
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorXin Jin
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorQin Peng
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Search for more papers by this authorCorresponding Author
Xiaofei Liu
Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin, 300072 People's Republic of China
Research Institute of Advanced Polymer, Tianjin University, Tianjin, 300072 People's Republic of China
Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300072 People's Republic of China
Correspondence to: X. F. Liu (E-mail: [email protected])Search for more papers by this authorYuntang Wu
Department of Health Statistics, College of Public Health, Tianjin Medical University, Tianjin, 300070 People's Republic of China
Search for more papers by this authorABSTRACT
Chitosan biguanidine hydrochloride (CSGH) was synthesized by guanidinylation of chitosan hydrochloride (CSH) under microwave irradiation and characterized by Fourier transform infrared spectroscopy, gel permeation chromatography, and element analysis. The antioxidant activity of CSGH was evaluated by both chemical and cellular methods. The results showed that CSGH was better than CSH but slightly inferior to ascorbic acid (Vc) for scavenging 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radicals, and the DPPH• scavenging ability of CSGH was dose-dependent. However, cell experiments indicated CSGH had a superior repairing effect on oxidatively damaged cells. The intervention of CSGH could excellently recover the cell morphology, decrease the level of malondialdehyde, and enhance the activity of superoxide dismutase and glutathione peroxidase. CSGH could be a potential antioxidant reagent, and its antioxidant activity was reflected not only in scavenging the free radicals but also in regulating the oxidation/antioxidation balance of damaged cells. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43570.
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