Functionalization of hybrid poly(n-isopropylacrylamide) hydrogels for Escherichia coli cell capture via adsorbed intermediate dye molecule
Jarod Gregory
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Search for more papers by this authorJonathon Cannell
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Search for more papers by this authorMatthew Kofron
Division of Developmental Biology, Cincinnati Children's Hospital and Medical Center, 3333 Burnet Ave., Cincinnati, Ohio, 45229
Search for more papers by this authorCorresponding Author
Lilit Yeghiazarian
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Correspondence to: L. Yeghiazarian (E-mail: [email protected]) and V. Nistor (E-mail: [email protected])Search for more papers by this authorCorresponding Author
Vasile Nistor
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Correspondence to: L. Yeghiazarian (E-mail: [email protected]) and V. Nistor (E-mail: [email protected])Search for more papers by this authorJarod Gregory
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Search for more papers by this authorJonathon Cannell
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Search for more papers by this authorMatthew Kofron
Division of Developmental Biology, Cincinnati Children's Hospital and Medical Center, 3333 Burnet Ave., Cincinnati, Ohio, 45229
Search for more papers by this authorCorresponding Author
Lilit Yeghiazarian
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Correspondence to: L. Yeghiazarian (E-mail: [email protected]) and V. Nistor (E-mail: [email protected])Search for more papers by this authorCorresponding Author
Vasile Nistor
Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, 2600 Clifton Ave., Cincinnati, Ohio, 45220
Correspondence to: L. Yeghiazarian (E-mail: [email protected]) and V. Nistor (E-mail: [email protected])Search for more papers by this authorABSTRACT
Poly(n-isopropylacrylamide) Laponite (PNIPAM-Lap) hybrid hydrogels, which use the synthetic clay Laponite as a crosslinker, permanently adsorb cationic laser dyes out of solution. This proof-of-concept expounds on this capability by adsorbing an intermediate dye molecule and using it as the foundation for successfully conjugating microbial antibodies to the surface of a PNIPAM hydrogel. The study involves using acriflavinium chloride molecules, adsorbed by a PNIPAM-Lap hydrogel from an acriflavine laser dye solution, as an intermediate molecule to attach antibodies raised against E. coli to the hydrogel and demonstrate cell capture. Furthermore, this system exemplifies a novel biotechnological platform for greatly expanding PNIPAM hydrogels' capabilities and applicability through conjugation chemistry to surface-bound molecules. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41557.
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March 10, 2015
