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Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) -jeffamine® hydrogel beads

Guohua Chen

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands

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Leen Van Der Does,

Corresponding Author

Leen Van Der Does

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands===Search for more papers by this author

Adriaan Bantjes,

Adriaan Bantjes

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands

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Guohua Chen,

Guohua Chen

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands

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Leen Van Der Does,

Corresponding Author

Leen Van Der Does

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands===Search for more papers by this author

Adriaan Bantjes,

Adriaan Bantjes

Department of Chemical Technology, Biomaterials Section, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands

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First published: 15 May 1993

https://doi.org/10.1002/app.1993.070480707

Citations: 21

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Abstract

Hydrogel-like polymers with reactive cyclic carbonate (CCA) groups have been obtained by the cross-linking reaction of poly(vinylene carbonate) (PVCA) with Jeffamines®. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use. © 1993 John Wiley & Sons, Inc.

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Volume48, Issue7

15 May 1993

Pages 1189-1198

Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) -jeffamine® hydrogel beads

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Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) -jeffamine® hydrogel beads

Abstract

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