Concurrent Prebiotic Formation of Nucleoside-Amidophosphates and Nucleoside-Triphosphates Potentiates Transition from Abiotic to Biotic Polymerization
Dr. Huacan Lin
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037 USA
NSF-NASA Center for Chemical Evolution, Atlanta, GA, 30332 USA
Search for more papers by this authorDr. Eddy I. Jiménez
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037 USA
Search for more papers by this authorJoshua T. Arriola
Department of Chemistry and Biochemistry, UC San Diego, 9500 Gilman Drive, La Jolla, CA, 92037 USA
Search for more papers by this authorCorresponding Author
Prof. Dr. Ulrich F. Müller
Department of Chemistry and Biochemistry, UC San Diego, 9500 Gilman Drive, La Jolla, CA, 92037 USA
Search for more papers by this authorCorresponding Author
Prof. Dr. Ramanarayanan Krishnamurthy
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037 USA
NSF-NASA Center for Chemical Evolution, Atlanta, GA, 30332 USA
Search for more papers by this authorDr. Huacan Lin
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037 USA
NSF-NASA Center for Chemical Evolution, Atlanta, GA, 30332 USA
Search for more papers by this authorDr. Eddy I. Jiménez
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037 USA
Search for more papers by this authorJoshua T. Arriola
Department of Chemistry and Biochemistry, UC San Diego, 9500 Gilman Drive, La Jolla, CA, 92037 USA
Search for more papers by this authorCorresponding Author
Prof. Dr. Ulrich F. Müller
Department of Chemistry and Biochemistry, UC San Diego, 9500 Gilman Drive, La Jolla, CA, 92037 USA
Search for more papers by this authorCorresponding Author
Prof. Dr. Ramanarayanan Krishnamurthy
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037 USA
NSF-NASA Center for Chemical Evolution, Atlanta, GA, 30332 USA
Search for more papers by this authorIn memory of Leslie Orgel
Graphical Abstract
The DAP mediated prebiotic phosphorylation of nucleosides/nucleotides/oligonucleotides produces a spectrum of the corresponding mono-, di-, and tri(amido)phosphorylated derivatives which are substrates for both prebiotic-oligomerization and biotic-polymerization/ligation. The compatibility of the phosphorylation and activation chemistry across a broad spectrum of (oligo)nucleos(t)ide structures suggests a smoother transition from chemistry to biology.
Abstract
Polymerization of nucleic acids in biology utilizes 5′-nucleoside triphosphates (NTPs) as substrates. The prebiotic availability of NTPs has been unresolved and other derivatives of nucleoside-monophosphates (NMPs) have been studied. However, this latter approach necessitates a change in chemistries when transitioning to biology. Herein we show that diamidophosphate (DAP), in a one-pot amidophosphorylation-hydrolysis setting converts NMPs into the corresponding NTPs via 5′-nucleoside amidophosphates (NaPs). The resulting crude mixture of NTPs are accepted by proteinaceous- and ribozyme-polymerases as substrates for nucleic acid polymerization. This phosphorylation also operates at the level of oligonucleotides enabling ribozyme-mediated ligation. This one-pot protocol for simultaneous generation of NaPs and NTPs suggests that the transition from prebiotic-phosphorylation and oligomerization to an enzymatic processive-polymerization can be more continuous than previously anticipated.
Conflict of interest
The authors declare no conflict of interest.
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