Volume 55, Issue 2 pp. 719-723

Communication

Dual-Targeting Nanovesicles for In Situ Intracellular Imaging of and Discrimination between Wild-type and Mutant p53

Ruocan Qian,

Ruocan Qian

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)

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Yue Cao,

Yue Cao

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)

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Prof. Yi-Tao Long,

Corresponding Author

Prof. Yi-Tao Long

[email protected]

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)Search for more papers by this author

Ruocan Qian,

Ruocan Qian

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)

Search for more papers by this author

Yue Cao,

Yue Cao

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)

Search for more papers by this author

Prof. Yi-Tao Long,

Corresponding Author

Prof. Yi-Tao Long

[email protected]

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)

Key Laboratory for Advanced Materials, East China University of Science and Technology, Shanghai, 200237 (P.R. China)Search for more papers by this author

First published: 27 November 2015

https://doi.org/10.1002/anie.201510142

Citations: 37

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Graphical Abstract

Wild and mutant imaging: Dual-targeting nanovesicles based on consensus DNA-functionalized plasmonic gold nanoparticles (AuNPs) are designed and used for the simultaneous plasmonic imaging of wild-type p53 and fluorescence imaging of mutant p53.

Abstract

p53 is a tumor-suppressor protein related to the cell cycle and programmed cell apoptosis. Herein, dual-targeting nanovesicles are designed for in situ imaging of intracellular wild-type p53 (WTp53) and mutant p53 (MUp53). Nanovesicle-encapsulated plasmonic gold nanoparticles (AuNPs) were functionalized with consensus DNA duplexes, and a fluorescein isothiocyanate (FITC)-marked anti-MUp53 antibody was conjugated to the nanovesicle surface. After entering the cytoplasm, the released AuNPs aggregated through recognition of WTp53 and the double-stranded DNA. The color changes of AuNPs were observed using dark-field microscopy, which showed the intracellular WTp53 distribution. The MUp53 location was detected though the immunological recognition between FITC-labeled anti-MUp53 and MUp53. Thus, a one-step incubation method for the in situ imaging of intracellular WTp53 and MUp53 was obtained; this was used to monitor the p53 level under a drug treatment.

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References

1
1aJ. X. Wang, X. Zhu, Q. Y. Tu, Q. Guo, C. S. Zarui, J. Momand, X. Z. Sun, F. M. Zhou, Anal. Chem. 2008, 80, 769–774;
10.1021/ac0714112
CAS PubMed Web of Science® Google Scholar
1bY. C. Wang, X. Zhu, M. H. Wu, N. Xia, J. X. Wang, F. M. Zhou, Anal. Chem. 2009, 81, 8441–8446;
10.1021/ac9014269
CAS PubMed Web of Science® Google Scholar
1cB. Vogelstein, D. Lane, A. J. Levine, Nature 2000, 408, 307–310.
10.1038/35042675
CAS PubMed Web of Science® Google Scholar
2
2aK. Iwabuchi, P. L. Bartel, B. Li, R. Marraccino, S. Fields, Proc. Natl. Acad. Sci. USA 1994, 91, 6098–6102;
10.1073/pnas.91.13.6098
CAS PubMed Web of Science® Google Scholar
2bT. Kanda, K. Segawa, N. Ohuchi, S. Mori, Y. Ito, Mol. Cell. Biol. 1994, 14, 2651–2663;
10.1128/MCB.14.4.2651
CAS PubMed Web of Science® Google Scholar
2cT. Unger, J. A. Mietz, M. Scheffner, C. L. Yee, P. M. Howley, Mol. Cell. Biol. 1993, 13, 5186–5194.
10.1128/MCB.13.9.5186
CAS PubMed Web of Science® Google Scholar
3
3aP. Wang, M. Reed, Y. Wang, G. Mayr, J. E. Stenger, M. E. Anderson, J. F. Schwedes, P. Tegtmeyer, Mol. Cell. Biol. 1994, 14, 5182–5191;
10.1128/MCB.14.8.5182
CAS PubMed Web of Science® Google Scholar
3bY. Wang, F. J. Schwedes, D. Parks, K. Mann, P. Tegtmeyer, Mol. Cell. Biol. 1995, 15, 2157–2165;
10.1128/MCB.15.4.2157
CAS PubMed Web of Science® Google Scholar
3cN. P. Pavletich, K. A. Chambers, C. O. Pabo, Genes Dev. 1993, 7, 2556–2564;
10.1101/gad.7.12b.2556
CAS PubMed Web of Science® Google Scholar
3dT. Gohler, S. Jager, G. Warnecke, H. Yasuda, E. Kim, W. Deppert, Nucleic Acids Res. 2005, 33, 1087–1100;
10.1093/nar/gki252
CAS PubMed Web of Science® Google Scholar
3eM. Reed, B. Woelker, P. Wang, Y. Wang, M. E. Anderson, P. Tegtmeyer, Proc. Natl. Acad. Sci. USA 1995, 92, 9455–9459.
10.1073/pnas.92.21.9455
CAS PubMed Web of Science® Google Scholar
4
4aA. J. Levine, Cell 1997, 88, 323–331;
10.1016/S0092-8674(00)81871-1
CAS PubMed Web of Science® Google Scholar
4bD. Hamroun, S. Kato, C. Ishioka, M. Claustres, C. Beroud, T. Soussi, Hum. Mutat. 2006, 27, 14–20;
10.1002/humu.20269
CAS PubMed Web of Science® Google Scholar
4cA. J. Levine, C. A. Finlay, P. W. Hinds, Cell 2004, 116, S 67–S69;
10.1016/S0092-8674(04)00036-4
CAS PubMed Google Scholar
4dT. Soussi, C. Ishioka, M. Claustres, C. Béroud, Nat. Rev. Cancer 2006, 6, 83–90;
10.1038/nrc1783
CAS PubMed Web of Science® Google Scholar
4eT. Soussi, J. M. Rubio-Nevado, C. Ishioka, Hum. Mutat. 2006, 27, 1151–1154.
10.1002/humu.20395
CAS PubMed Web of Science® Google Scholar
5
5aL. Bai, W. Zhu, J. Cancer Mol. 2006, 2, 141–153;
CAS Google Scholar
5bC. Xiang, C. Shen, Z. Wu, Y. Qin, Y. Zhang, C. Liu, J. Chen, S. Zhang, J. Occup. Health 2007, 49, 279–284.
10.1539/joh.49.279
CAS PubMed Web of Science® Google Scholar
6
6aE. Jagelská, V. Brázda, S. Pospisilová, B. Vojtesek, E. Palecek, J. Immunol. Methods 2002, 267, 227–235;
10.1016/S0022-1759(02)00182-5
PubMed Web of Science® Google Scholar
6bK. A. Gould, C. Nixon, M. J. Tilby, Mol. Pharmacol. 2004, 66, 1301–1309.
10.1124/mol.104.000596
CAS PubMed Web of Science® Google Scholar
7
7aI. I. Wistuba, A. F. Gazdar, I. Roa, J. Albores-Saavedra, Hum. Pathol. 1996, 27, 360–365;
10.1016/S0046-8177(96)90109-4
PubMed Web of Science® Google Scholar
7bE. T. M. A. Gaballah, M. A. Tawfik, Saudi Dent. J. 2010, 22, 167–170;
10.1016/j.sdentj.2010.07.002
PubMed Google Scholar
7cM. H. Bukhari, S. Niazi, M. Anwar, N. A. Chaudhry, S. Naeem, Ann. N. Y. Acad. Sci. 2008, 1138, 1–9.
10.1196/annals.1414.001
CAS PubMed Web of Science® Google Scholar
8
8aY. Adachi, W. Chen, H. S. Wei, T. Kamata, Anal. Biochem. 2005, 342, 348–351;
10.1016/j.ab.2005.03.056
CAS PubMed Web of Science® Google Scholar
8bI. Beno, K. Rosenthal, M. Levitine, L. Shaulov, T. E. Haran, Nucleic Acids Res. 2011, 39, 1919–1932.
10.1093/nar/gkq1044
CAS PubMed Web of Science® Google Scholar
9X. L. Chen, C. H. He, Z. F. Zhang, J. X. Wang, Biosens. Bioelectron. 2013, 47, 335–339.
10.1016/j.bios.2013.03.059
CAS PubMed Web of Science® Google Scholar
10
10aJ. N. Anker, W. P. Hall, O. Lyandres, N. C. Shah, J. Zhao, R. P. VanDuyne, Nat. Mater. 2008, 7, 442–453;
10.1038/nmat2162
CAS PubMed Web of Science® Google Scholar
10bS. Eustis, M. A. El-Sayed, Chem. Soc. Rev. 2006, 35, 209;
10.1039/B514191E
CAS PubMed Web of Science® Google Scholar
10cK. R. Catchpole, A. Polman, Opt. Express 2008, 16, 21793–21800;
10.1364/OE.16.021793
CAS PubMed Web of Science® Google Scholar
10dK. M. Mayer, J. H. Hafner, Chem. Rev. 2011, 111, 3828.
10.1021/cr100313v
CAS PubMed Web of Science® Google Scholar
11Y. Li, C. Jing, L. Zhang, Y. T. Long, Chem. Soc. Rev. 2012, 41, 632–642.
10.1039/C1CS15143F
CAS PubMed Web of Science® Google Scholar
12
12aL. X. Qin, C. Jing, Y. Li, D. W. Li, Y. T. Long, Chem. Commun. 2012, 48, 1511–1513;
10.1039/C1CC14326C
CAS PubMed Web of Science® Google Scholar
12bQ. Liu, C. Jing, X. X. Zheng, Z. Gu, D. Li, D. W. Li, Q. Huang, Y. T. Long, C. H. Fan, Chem. Commun. 2012, 48, 9574–9576.
10.1039/c2cc34632j
CAS PubMed Web of Science® Google Scholar
13C. Jing, Z. Gu, Y. L. Ying, D. W. Li, L. Zhang, Y. T. Long, Anal. Chem. 2012, 84, 4284–4291.
10.1021/ac203118g
CAS PubMed Web of Science® Google Scholar
14D. S. Seferos, D. A. Giljohann, H. D. Hill, A. E. Prigodich, C. A. Mirkin, J. Am. Chem. Soc. 2007, 129, 15477–15479.
10.1021/ja0776529
CAS PubMed Web of Science® Google Scholar
15B. Dalby, S. Cates, A. Harris, E. C. Ohki, M. L. Tilkins, P. J. Price, V. C. Ciccarone, Methods 2004, 33, 95–103.
10.1016/j.ymeth.2003.11.023
CAS PubMed Web of Science® Google Scholar
16M. M. Elmi, M. N. Sarbolouki, Int. J. Pharm. 2001, 215, 45–50.
10.1016/S0378-5173(00)00667-0
CAS PubMed Web of Science® Google Scholar
17
17aK. C. Grabar, R. G. Freeman, M. B. Hommer, M. J. Natan, Anal. Chem. 1995, 67, 735–743;
10.1021/ac00100a008
CAS Web of Science® Google Scholar
17bS. Link, M. A. El-Sayed, J. Phys. Chem. B 1999, 103, 4212–4217.
10.1021/jp984796o
CAS Web of Science® Google Scholar
18L. T. Vassilev, B. T. Vu, B. Graves, D. Carvajal, F. Podlaski, Z. Filipovic, N. Kong, U. Kammlott, C. Lukacs, C. Klein, N. Fotouhi, E. A. Liu, Science 2004, 303, 844–848.
10.1126/science.1092472
CAS PubMed Web of Science® Google Scholar

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Volume55, Issue2

January 11, 2016

Pages 719-723

Dual-Targeting Nanovesicles for In Situ Intracellular Imaging of and Discrimination between Wild-type and Mutant p53

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Abstract

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Dual-Targeting Nanovesicles for In Situ Intracellular Imaging of and Discrimination between Wild-type and Mutant p53

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