Structure of the Parallel Duplex of Poly(A) RNA: Evaluation of a 50 Year-Old Prediction†
Corresponding Author
Nozhat Safaee
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)Search for more papers by this authorDr. Anne M. Noronha
Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, H4B 1R6 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorDmitry Rodionov
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorDr. Guennadi Kozlov
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorProf. Christopher J. Wilds
Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, H4B 1R6 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorProf. George M. Sheldrick
Department of Structural Chemistry, University of Göttingen, 37077 Göttingen (Germany)
Search for more papers by this authorCorresponding Author
Prof. Kalle Gehring
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)Search for more papers by this authorCorresponding Author
Nozhat Safaee
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)Search for more papers by this authorDr. Anne M. Noronha
Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, H4B 1R6 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorDmitry Rodionov
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorDr. Guennadi Kozlov
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorProf. Christopher J. Wilds
Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, H4B 1R6 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Search for more papers by this authorProf. George M. Sheldrick
Department of Structural Chemistry, University of Göttingen, 37077 Göttingen (Germany)
Search for more papers by this authorCorresponding Author
Prof. Kalle Gehring
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)
Groupe de recherche axé sur la structure des protéines McGill University, Montréal, Québec, H3G 0B1 (Canada)
Department of Biochemistry, McGill University, Montréal, Québec, H3G 0B1 (Canada)Search for more papers by this authorPrimary data collection was done at McGill University. Additional data was collected by Pawel Grochulski and Shaunivan Labiuk at the Canadian Light Source, Saskatoon, SK, Canada and at the Cornell High Energy Synchrotron Source, Ithaca, NY (USA).
Graphical Abstract
The other double helix: The molecular structure of double-stranded poly(rA) was predicted in 1961, but it has only now been confirmed. The crystal structure of the parallel double helix of (rA)11 obtained at physiological pH was solved using ab initio direct methods and refined to 1.0 Å resolution. The crystals have bound ammonium ions that are complexed by the RNA phosphates and adenine N1 atoms (see section of the structure).
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