Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad-Spectrum Nanoquencher†
Dr. Xinglu Huang
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorMagdalena Swierczewska
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Department of Biomedical Engineering, Stony Brook University (USA)
Search for more papers by this authorDr. Ki Young Choi
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorDr. Lei Zhu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorDr. Ashwinkumar Bhirde
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorDr. Kwangmeyung Kim
Center for Theragnosis, Korea Institute of Science and Technology (KIST, Korea)
Search for more papers by this authorProf. Jin Xie
Department of Chemistry and Bio-Imaging Research Center, University of Georgia (USA)
Search for more papers by this authorDr. Gang Niu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorProf. Kang Choon Lee
College of Pharmacy, SungKyunKwan University (Korea)
Search for more papers by this authorCorresponding Author
Dr. Seulki Lee
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)Search for more papers by this authorCorresponding Author
Dr. Xiaoyuan Chen
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)Search for more papers by this authorDr. Xinglu Huang
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorMagdalena Swierczewska
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Department of Biomedical Engineering, Stony Brook University (USA)
Search for more papers by this authorDr. Ki Young Choi
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorDr. Lei Zhu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorDr. Ashwinkumar Bhirde
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorDr. Kwangmeyung Kim
Center for Theragnosis, Korea Institute of Science and Technology (KIST, Korea)
Search for more papers by this authorProf. Jin Xie
Department of Chemistry and Bio-Imaging Research Center, University of Georgia (USA)
Search for more papers by this authorDr. Gang Niu
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Search for more papers by this authorProf. Kang Choon Lee
College of Pharmacy, SungKyunKwan University (Korea)
Search for more papers by this authorCorresponding Author
Dr. Seulki Lee
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)Search for more papers by this authorCorresponding Author
Dr. Xiaoyuan Chen
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)Search for more papers by this authorThis work was supported by the Intramural Research Program of the NIBIB, NIH and partially supported by an NIH Pathway to Independence (K99/R00) Award, the Henry M. Jackson Foundation, and the Intramural Research Program of KIST (Korea). We thank Myung Sun Lee for illustrations.
Graphical Abstract
Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).
Supporting Information
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