Volume 46, Issue 12 pp. 1992-1994

Communication

Redox Potentiometry Studies of Particulate Methane Monooxygenase: Support for a Trinuclear Copper Cluster Active Site^†

Sunney I. Chan Prof.,

Sunney I. Chan Prof.

[email protected]

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

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Vincent C.-C. Wang,

Vincent C.-C. Wang

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Department of Chemistry, National Taiwan University, Taipei 106, Taiwan, Republic of China

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Jeff C.-H. Lai,

Jeff C.-H. Lai

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

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Steve S.-F. Yu,

Steve S.-F. Yu

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

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Peter P.-Y. Chen,

Peter P.-Y. Chen

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

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Kelvin H.-C. Chen,

Kelvin H.-C. Chen

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

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Chang-Li Chen,

Chang-Li Chen

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

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Michael K. Chan Prof.,

Michael K. Chan Prof.

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Departments of Chemistry and Biochemistry, Ohio State University, Columbus, OH 43210, USA

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Sunney I. Chan Prof.,

Sunney I. Chan Prof.

[email protected]

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

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Vincent C.-C. Wang,

Vincent C.-C. Wang

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Department of Chemistry, National Taiwan University, Taipei 106, Taiwan, Republic of China

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Jeff C.-H. Lai,

Jeff C.-H. Lai

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Search for more papers by this author

Steve S.-F. Yu,

Steve S.-F. Yu

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Search for more papers by this author

Peter P.-Y. Chen,

Peter P.-Y. Chen

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Search for more papers by this author

Kelvin H.-C. Chen,

Kelvin H.-C. Chen

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Search for more papers by this author

Chang-Li Chen,

Chang-Li Chen

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Search for more papers by this author

Michael K. Chan Prof.,

Michael K. Chan Prof.

Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China, Fax: (+886) 2-2783-1237

Departments of Chemistry and Biochemistry, Ohio State University, Columbus, OH 43210, USA

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First published: 02 March 2007

https://doi.org/10.1002/anie.200604647

Citations: 134

^†

This work was supported by Academia Sinica as well as by a grant from the National Science Council (NSC94-2113-M-001-046).

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Graphical Abstract

A pocketful of coppers: Redox potentiometry and EPR experiments have confirmed that the active site of the particulate methane monooxygenase (pMMO), a membrane-bound enzyme that hydroxylates methane to methanol under ambient conditions, consists of one trinuclear copper cluster (see picture) and one type 2 copper site, in addition to the dinuclear copper cluster revealed previously by X-ray crystallography.

Supporting Information

References

1S. S. F. Yu, K. H. C. Chen, M. Y. H. Tseng, C. F. Tseng, Y. S. Wang, Y. J. Chen, D. H. Huang, S. I. Chan, J. Bacteriol. 2003, 185, 5915–5924.
10.1128/JB.185.20.5915-5924.2003
CAS PubMed Web of Science® Google Scholar
2H. H. T. Nguyen, S. J. Elliott, J. H. K. Yip, S. I. Chan, J. Biol. Chem. 1998, 273, 7957–7966.
10.1074/jbc.273.14.7957
CAS PubMed Web of Science® Google Scholar
3H. H. T. Nguyen, K. H. Nakagawa, B. Hedman, S. J. Elliott, M. E. Lidstrom, K. O. Hodgson, S. I. Chan, J. Am. Chem. Soc. 1996, 118, 12766–12776.
10.1021/ja961778g
CAS Web of Science® Google Scholar
4S. I. Chan, K. H. C. Chen, S. S. F. Yu, C. L. Chen, S. S. J. Kuo, Biochemistry 2004, 43, 4421–4430.
10.1021/bi0497603
CAS PubMed Web of Science® Google Scholar
5K. H. C. Chen, C. L. Chen, C. F. Tseng, S. S. F. Yu, S. C. Ke, J. F. Lee, H. H. T. Nguyen, S. J. Elliott, J. O. Alben, S. I. Chan, J. Chin. Chem. Soc. 2004, 51, 1081–1098.
10.1002/jccs.200400162
CAS Web of Science® Google Scholar
6P. Basu, B. Katterle, K. K. Andersson, H. Dalton, Biochem. J. 2003, 369, 417–427.
10.1042/BJ20020823
CAS PubMed Web of Science® Google Scholar
7D. W. Choi, R. C. Kunz, E. S. Boyd, J. D. Semrau, W. E. Antholine, J. I. Han, J. A. Zahn, J. M. Boyd, A. M. Mora, A. A. DiSpirito, J. Bacteriol. 2003, 185, 5755–5764.
10.1128/JB.185.19.5755-5764.2003
CAS PubMed Web of Science® Google Scholar
8R. L. Lieberman, D. B. Shrestha, P. E. Doan, B. M. Hoffman, T. L. Stemmler, A. C. Rosenzweig, Proc. Natl. Acad. Sci. USA 2003, 100, 3820–3825.
10.1073/pnas.0536703100
CAS PubMed Web of Science® Google Scholar
9R. L. Lieberman, A. C. Rosenzweig, Crit. Rev. Biochem. Mol. Biol. 2004, 39, 147–164.
10.1080/10409230490475507
CAS PubMed Web of Science® Google Scholar
10M. Takeguchi, K. Miyakawa, I. Okura, Biometals 1998, 11, 229–234.
10.1023/A:1009278216452
CAS PubMed Web of Science® Google Scholar
11R. L. Lieberman, A. C. Rosenzweig, Nature 2005, 434, 177–182.
10.1038/nature03311
CAS PubMed Web of Science® Google Scholar
12R. L. Lieberman, A. C. Rosenzweig, Dalton Trans. 2005, 3390–3396.
10.1039/b506651d
CAS PubMed Web of Science® Google Scholar
13S. C. Hung, C. L. Chen, K. H. C. Chen, S. S. F. Yu, S. I. Chan, J. Chin. Chem. Soc. 2004, 51, 1229–1244.
10.1002/jccs.200400179
CAS Web of Science® Google Scholar
14P. P. Y. Chen, R. B. G. Yang, S. I. Chan, unpublished results.
Google Scholar
15A. T. Brunger, P. D. Adams, G. M. Clore, W. K. Delano, P. Gros, R. W. Grosse-Kunstleve, J.-S. Jiang, J. Kuszewski, M. Nilges, N. S. Pannu, R. J. Read, L. L. M. Rice, T. Simonson, G. L. Warren, Acta Crystallogr. Sect. D 1998, 54, 905–921.
10.1107/S0907444998003254
CAS PubMed Web of Science® Google Scholar

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Volume46, Issue12

March 12, 2007

Pages 1992-1994

Redox Potentiometry Studies of Particulate Methane Monooxygenase: Support for a Trinuclear Copper Cluster Active Site^†

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Redox Potentiometry Studies of Particulate Methane Monooxygenase: Support for a Trinuclear Copper Cluster Active Site†

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Redox Potentiometry Studies of Particulate Methane Monooxygenase: Support for a Trinuclear Copper Cluster Active Site^†