Volume 46, Issue 12 pp. 1999-2001

Communication

Detecting Force-Induced Molecular Transitions with Fluorescence Resonant Energy Transfer^†

Peter B. Tarsa Dr.

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Dr. P. B. Tarsa, R. R. Brau, and M. Barch contributed equally to this work.

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Ricardo R. Brau,

Ricardo R. Brau

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Dr. P. B. Tarsa, R. R. Brau, and M. Barch contributed equally to this work.

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Mariya Barch,

Mariya Barch

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA

Dr. P. B. Tarsa, R. R. Brau, and M. Barch contributed equally to this work.

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Jorge M. Ferrer,

Jorge M. Ferrer

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

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Yelena Freyzon,

Yelena Freyzon

Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA

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Paul Matsudaira Prof.,

Paul Matsudaira Prof.

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

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Matthew J. Lang Prof.,

Matthew J. Lang Prof.

[email protected]

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Mechanical Engineering Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

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Peter B. Tarsa Dr.,

Peter B. Tarsa Dr.

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Dr. P. B. Tarsa, R. R. Brau, and M. Barch contributed equally to this work.

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Ricardo R. Brau,

Ricardo R. Brau

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Dr. P. B. Tarsa, R. R. Brau, and M. Barch contributed equally to this work.

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Mariya Barch,

Mariya Barch

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA

Dr. P. B. Tarsa, R. R. Brau, and M. Barch contributed equally to this work.

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Jorge M. Ferrer,

Jorge M. Ferrer

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

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Yelena Freyzon,

Yelena Freyzon

Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA

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Paul Matsudaira Prof.,

Paul Matsudaira Prof.

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

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Matthew J. Lang Prof.,

Matthew J. Lang Prof.

[email protected]

Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, Fax: (+1) 617-324-7554 http://web.mit.edu/langlab

Mechanical Engineering Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

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First published: 02 March 2007

https://doi.org/10.1002/anie.200604546

Citations: 55

^†

Support was provided by a National Institutes of Health National Research Service Award (P.B.T.), the MIT/NIGMS Biotechnology Training Program (R.R.B.), the Singapore–MIT Alliance (M.B., Y.F., P.M.), the National Institutes of Health (J.M.F.), the National Science Foundation (P.M.), and the W.M. Keck Foundation (M.J.L.).

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Graphical Abstract

Don't FRET: The first successful combination of optical-tweezers force microscopy and single-molecule fluorescence resonant energy transfer (FRET) is demonstrated with a force sensor based on a DNA hairpin (see picture). As the hairpin is opened and closed by the optical tweezers, the structural change is simultaneously monitored by the FRET emission from fluorescence labels.

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Volume46, Issue12

March 12, 2007

Pages 1999-2001

Detecting Force-Induced Molecular Transitions with Fluorescence Resonant Energy Transfer^†

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Detecting Force-Induced Molecular Transitions with Fluorescence Resonant Energy Transfer^†