Turning Inhibitors into Activators: A Hammerhead Ribozyme Controlled by a Guanine Quadruplex†
Markus Wieland
University of Konstanz, Department of Chemistry, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-885-140
Search for more papers by this authorJörg S. Hartig Prof. Dr.
University of Konstanz, Department of Chemistry, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-885-140
Search for more papers by this authorMarkus Wieland
University of Konstanz, Department of Chemistry, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-885-140
Search for more papers by this authorJörg S. Hartig Prof. Dr.
University of Konstanz, Department of Chemistry, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-885-140
Search for more papers by this authorJ.S.H. gratefully acknowledges the Volkswagen Foundation for funding a Lichtenberg Professorship. We thank the Department of Chemistry at the University of Konstanz for support.
Graphical Abstract
Modular design of functional RNAs can be used to tailor molecular properties. Guanosine-rich sequences were introduced as switching devices for functional nucleic acids. TMPyP4 (see picture) was identified as the strongest ribozyme inhibitor known to date. When quadruplex-forming, G-rich sequences were attached to the ribozyme, the TMPyP4-mediated response was inverted from inhibition to activation of the ribozyme reaction.
Supporting Information
Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z600909_s.pdf or from the author.
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