Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery†
Davide Pantarotto Dipl.-Chem.
Dipartimento di Scienze Farmaceutiche, Università di Trieste, 34127 Trieste, Italy, Fax: (+39) 040-5272
Institut de Biologie Moléculaire et Cellulaire, UPR9021 CNRS, Immunologie et Chimie Thérapeutiques, 67084 Strasbourg, France, Fax: (+33) 388-610-680
Search for more papers by this authorRavi Singh Dipl.-Chem.
Centre for Drug Delivery Research and Electron Microscopy Unit, The School of Pharmacy, University of London, London WC1N 1AX, United Kingdom, Fax: (+39) 207-7535942
Search for more papers by this authorDavid McCarthy Dipl.-Chem.
Centre for Drug Delivery Research and Electron Microscopy Unit, The School of Pharmacy, University of London, London WC1N 1AX, United Kingdom, Fax: (+39) 207-7535942
Search for more papers by this authorMathieu Erhardt Dr.
Institut de Biologie Moléculaire des Plantes, 67084 Strasbourg, France
Search for more papers by this authorJean-Paul Briand Dr.
Institut de Biologie Moléculaire et Cellulaire, UPR9021 CNRS, Immunologie et Chimie Thérapeutiques, 67084 Strasbourg, France, Fax: (+33) 388-610-680
Search for more papers by this authorMaurizio Prato Prof.
Dipartimento di Scienze Farmaceutiche, Università di Trieste, 34127 Trieste, Italy, Fax: (+39) 040-5272
Search for more papers by this authorKostas Kostarelos Dr.
Centre for Drug Delivery Research and Electron Microscopy Unit, The School of Pharmacy, University of London, London WC1N 1AX, United Kingdom, Fax: (+39) 207-7535942
Search for more papers by this authorAlberto Bianco Dr.
Institut de Biologie Moléculaire et Cellulaire, UPR9021 CNRS, Immunologie et Chimie Thérapeutiques, 67084 Strasbourg, France, Fax: (+33) 388-610-680
Search for more papers by this authorDavide Pantarotto Dipl.-Chem.
Dipartimento di Scienze Farmaceutiche, Università di Trieste, 34127 Trieste, Italy, Fax: (+39) 040-5272
Institut de Biologie Moléculaire et Cellulaire, UPR9021 CNRS, Immunologie et Chimie Thérapeutiques, 67084 Strasbourg, France, Fax: (+33) 388-610-680
Search for more papers by this authorRavi Singh Dipl.-Chem.
Centre for Drug Delivery Research and Electron Microscopy Unit, The School of Pharmacy, University of London, London WC1N 1AX, United Kingdom, Fax: (+39) 207-7535942
Search for more papers by this authorDavid McCarthy Dipl.-Chem.
Centre for Drug Delivery Research and Electron Microscopy Unit, The School of Pharmacy, University of London, London WC1N 1AX, United Kingdom, Fax: (+39) 207-7535942
Search for more papers by this authorMathieu Erhardt Dr.
Institut de Biologie Moléculaire des Plantes, 67084 Strasbourg, France
Search for more papers by this authorJean-Paul Briand Dr.
Institut de Biologie Moléculaire et Cellulaire, UPR9021 CNRS, Immunologie et Chimie Thérapeutiques, 67084 Strasbourg, France, Fax: (+33) 388-610-680
Search for more papers by this authorMaurizio Prato Prof.
Dipartimento di Scienze Farmaceutiche, Università di Trieste, 34127 Trieste, Italy, Fax: (+39) 040-5272
Search for more papers by this authorKostas Kostarelos Dr.
Centre for Drug Delivery Research and Electron Microscopy Unit, The School of Pharmacy, University of London, London WC1N 1AX, United Kingdom, Fax: (+39) 207-7535942
Search for more papers by this authorAlberto Bianco Dr.
Institut de Biologie Moléculaire et Cellulaire, UPR9021 CNRS, Immunologie et Chimie Thérapeutiques, 67084 Strasbourg, France, Fax: (+33) 388-610-680
Search for more papers by this authorThis work was supported by the Centre National de la Recherche Scientifique (CNRS), Università di Trieste, and Ministero dell'Istruzione, dell' Università e della Ricerca (MIUR; cofin 2002, prot. 2002032171). Transmission electron microscopy (TEM) analysis was performed at the microscopy facility of the Institute of Biomedical Problems and was cofinanced by CNRS, Région Alsace, Louis Pasteur University, and the Association de la Recherche pour le Cancer. The authors wish to acknowledge C. D. Partidos for helpful and stimulating discussions. We thank Mr. Claudio Gamboz (Centro Servizi Polivalenti di Ateneo (CSPA), Università di Trieste) for his great help with the TEM measurements.
Graphical Abstract
Genetic vaccination and gene therapy research could benefit from the application of carbon nanotubes. Functionalized, positively charged, water-soluble carbon nanotubes are able to penetrate into cells (see figure) and can transport plasmid DNA by formation of noncovalent DNA–nanotube complexes. Such nanotubes can be used as novel nonviral delivery systems for gene transfer.
Supporting Information
Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z460437_s.pdf or from the author.
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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