A Universal Photoactivatable Tag Attached to Fluorophores Enables Their Use for Single-Molecule Imaging
Dr. Xuebo Zhang
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorDaoming Guan
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorYawei Liu
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorJinyang Liu
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorKuangshi Sun
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorSong Chen
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorYanxin Zhang
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorBingjie Zhao
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorTianli Zhai
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorCorresponding Author
Prof. Yunxiang Zhang
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorCorresponding Author
Qian Liu
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorDr. Xuebo Zhang
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorDaoming Guan
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorYawei Liu
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorJinyang Liu
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorKuangshi Sun
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorSong Chen
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorYanxin Zhang
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorBingjie Zhao
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorTianli Zhai
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorCorresponding Author
Prof. Yunxiang Zhang
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorCorresponding Author
Qian Liu
Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438 P. R. China
Search for more papers by this authorAbstract
Single molecule localization microscopy based on photoactivation is a powerful tool for investigating the ultrastructure of cells. We developed a general strategy for photoactivatable fluorophores, using 2,3-dihydro-1,4-oxathiine group (SO) as a tag to attach to various skeletal structures, including coumarin, BODIPY, rhodamine, and cyanine. The conjugation of SO resulted in a significant loss of fluorescence due to photoinduced electron transfer (PeT). Under the irradiation of excitation light, singlet oxygen generated by the fluorophores converted the SO moiety into its ester derivative, terminated the PeT process, and restored the fluorescence. Single molecule localization imaging was achieved using a dual functional illuminating beam in the visible, acting as both the activating and the exciting source. We successfully applied these photoactivatable probes for time-lapse super-resolution tracking in living cells and super-resolution imaging of microtubule structures in neurons.
Conflict of interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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| ange202211767-sup-0001-Photoactivated_cell_imaging.mp439.2 MB | Supporting Information |
| ange202211767-sup-0001-Time-lapse_super-resolution_tracking.mp45.8 MB | Supporting Information |
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