Isolation of genes from the Batten candidate region using exon amplification
Corresponding Author
T. J. Lerner
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Neurology Department, Harvard Medical School, Boston, Massachusetts
Molecular Neurogenetics Unit, The Massachusetts General Hospital, Building 149, 13th St., Charlestown, MA 02129Search for more papers by this authorK. L. D'Arigo
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Search for more papers by this authorJ. L. Haines
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Neurology Department, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorN. A. Doggett
Division of Life Sciences, Los Alamos National Laboratory, Los Alamos, New Mexico
Search for more papers by this authorP. E. M. Taschner
Department of Genetics, Leiden University, The Netherlands
Search for more papers by this authorN. de Vos
Department of Genetics, Leiden University, The Netherlands
Search for more papers by this authorA. J. Buckler
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Neurology Department, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorCorresponding Author
T. J. Lerner
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Neurology Department, Harvard Medical School, Boston, Massachusetts
Molecular Neurogenetics Unit, The Massachusetts General Hospital, Building 149, 13th St., Charlestown, MA 02129Search for more papers by this authorK. L. D'Arigo
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Search for more papers by this authorJ. L. Haines
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Neurology Department, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorN. A. Doggett
Division of Life Sciences, Los Alamos National Laboratory, Los Alamos, New Mexico
Search for more papers by this authorP. E. M. Taschner
Department of Genetics, Leiden University, The Netherlands
Search for more papers by this authorN. de Vos
Department of Genetics, Leiden University, The Netherlands
Search for more papers by this authorA. J. Buckler
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, New Mexico
Neurology Department, Harvard Medical School, Boston, Massachusetts
Search for more papers by this authorAbstract
In order to identify genes originating from the Batten disease candidate region, we have used the technique of exon amplification to identify transcribed sequences. This procedure produces trapped exon clones, which can represent single exons or multiple exons spliced together and is an efficient method for obtaining probes for physical mapping and for screening cDNA libraries. The source of DNA for these experiments was a collection of chromosome 16 cosmid contigs isolated by the direct subcloning of region-specific yeast artificial chromosomes (YACs) and hybridization of inter-alu PCR products from these YACs to the flow-sorted Los Alamos chromosome 16 cosmid library. We are now using the resulting exon probes to screen retina and brain cDNA libraries for candidate JNCL genes. © 1995 Wiley-Liss, Inc.
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